| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Medicine Branch, National Cancer Institute, NIH, Bethesda, Maryland 20878
To investigate if the estrogen control of the tumorigenic phenotype of breast cancer cells was mediated through activation of the c-fos protooncogene, we examined the expression of this oncogene in MCF-7 cells. In cells synchronized by double thymidine blockade, the peptide growth factors transforming growth factor 
and epidermal growth factor increased c-fos mRNA levels 6-fold above controls after 30 min of treatment. The phorbol ester, 12-O-tetradecanoylphorbol-13-acetate, increased c-fos mRNA levels 4- to 5-fold above control. 17ß-Estradiol, a growth stimulator, increased c-fos mRNA levels less than 2-fold above control levels, while progesterone, vitamin D3, dihydrotestosterone, and dexamethasone had little effect on c-fos mRNA levels. In contrast, 17ß-estradiol treatment initially diminished the c-myc RNA level after 30 min of treatment and resulted in an elevation of c-myc by 2.5 h after initiation of treatment. We conclude that c-fos induction in these cells is growth related and accompanies stimulation by transforming growth factor and epidermal growth factor. 17ß-Estradiol, on the other hand, induced much smaller increases in c-fos mRNA levels, suggesting an alternative or more complex mechanism of cellular stimulation.
1 To whom requests for reprints should be addressed.
Received 6/ 8/87. Revised 11/13/87. Accepted 11/16/87.
This article has been cited by other articles:
|
|
 |
|
  M. Maggiolini, A. Vivacqua, G. Fasanella, A. G. Recchia, D. Sisci, V. Pezzi, D. Montanaro, A. M. Musti, D. Picard, and S. Ando The G Protein-coupled Receptor GPR30 Mediates c-fos Up-regulation by 17{beta}-Estradiol and Phytoestrogens in Breast Cancer Cells J. Biol. Chem., June 25, 2004; 279(26): 27008 - 27016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Havrilesky, C. P. McMahon, E. K. Lobenhofer, R. Whitaker, J. R. Marks, and A. Berchuck Relationship Between Expression of Coactivators and Corepressors of Hormone Receptors and Resistance of Ovarian Cancers to Growth Regulation by Steroid Hormones Reproductive Sciences, April 1, 2001; 8(2): 104 - 113. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Duan, W. Porter, I. Samudio, C. Vyhlidal, M. Kladde, and S. Safe Transcriptional Activation of c-fos Protooncogene by 17{beta}-Estradiol: Mechanism of Aryl Hydrocarbon Receptor-Mediated Inhibition Mol. Endocrinol., September 1, 1999; 13(9): 1511 - 1521. [Abstract] [Full Text]
|
|
|
|
 |
|
 U Moens, M Van Ghelue, B Johansen, and O. Seternes Concerted expression of BK virus large T- and small t-antigens strongly enhances oestrogen receptor-mediated transcription J. Gen. Virol., March 1, 1999; 80(3): 585 - 594. [Abstract]
|
|
|
|
 |
|
 R. Duan, W. Porter, and S. Safe Estrogen-Induced c-fos Protooncogene Expression in MCF-7 Human Breast Cancer Cells: Role of Estrogen Receptor Sp1 Complex Formation Endocrinology, April 1, 1998; 139(4): 1981 - 1990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Duan, W. Xie, R. C. Burghardt, and S. Safe Estrogen Receptor-mediated Activation of the Serum Response Element in MCF-7 Cells through MAPK-dependent Phosphorylation of Elk-1 J. Biol. Chem., April 6, 2001; 276(15): 11590 - 11598. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
