This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Weinstock, J. Articles by Baldwin, G. S. Search for Related Content PubMed PubMed Citation Articles by Weinstock, J. Articles by Baldwin, G. S.

Binding of Gastrin₁₇ to Human Gastric Carcinoma Cell Lines

Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, P. O. Royal Melbourne Hospital, Victoria, Australia, 3050

The hormone gastrin stimulates acid secretion by gastric parietal cells and acts as a growth factor for the gastric mucosa. Gastrin receptors with dissociation constants of approximately 0.5 nM have been detected on isolated gastric parietal cells, and on some cell lines derived from colon carcinomas. We now report that gastrin is also bound by five cell lines derived from human gastric carcinomas, but that the affinities of these lines for gastrin range from 0.2 to 1.3 µM. Cholecystokinin₈ binds to the cell line Okajima with an affinity similar to gastrin₁₇, while shorter gastrin analogues bind with reduced affinity. Binding of gastrin is unaffected by acetylcholine, histamine, or a number of other hormones with the exception of insulin which inhibits binding with an IC₅₀ value of 0.5 µM. The ability to bind gastrin with affinities in the µM range appears to be a property widespread among other tumor cell lines.

¹ To whom requests for reprints should be addressed.

Received 1/13/87. Revised 9/ 2/87. Revised 11/11/87. Accepted 11/17/87.

This article has been cited by other articles:

				R. P. Thomas, M. R. Hellmich, C. M. Townsend Jr., and B. M. Evers Role of Gastrointestinal Hormones in the Proliferation of Normal and Neoplastic Tissues Endocr. Rev., October 1, 2003; 24(5): 571 - 599. [Abstract] [Full Text] [PDF]

				J.-C. Schaer and J. C. Reubi High Gastrin and Cholecystokinin (CCK) Gene Expression in Human Neuronal, Renal, and Myogenic Stem Cell Tumors: Comparison with CCK-A and CCK-B Receptor Contents J. Clin. Endocrinol. Metab., January 1, 1999; 84(1): 233 - 239. [Abstract] [Full Text]

				J. F. REHFELD The New Biology of Gastrointestinal Hormones Physiol Rev, October 1, 1998; 78(4): 1087 - 1108. [Abstract] [Full Text] [PDF]

				G S BALDWIN and A SHULKES Gastrin, gastrin receptors and colorectal carcinoma Gut, April 1, 1998; 42(4): 581 - 584. [Full Text] [PDF]

				P. Singh, A. Owlia, R. Espeijo, and B. Dai Novel Gastrin Receptors Mediate Mitogenic Effects of Gastrin and Processing Intermediates of Gastrin on Swiss 3T3 Fibroblasts J. Biol. Chem., April 14, 1995; 270(15): 8429 - 8438. [Abstract] [Full Text] [PDF]