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Departments of Cell Biology [W. S. K., P. F.] and Medicine [P. F.], The University of Texas M. D. Anderson Hospital and Tumor Institute at Houston, Houston, Texas 77030
Luria-Delbrück fluctuation analysis provides a method to estimate mutation rates in cell populations. Originally designed for bacterial populations, the method now is widely applied in somatic cell genetics and in cancer biology. However, there are fundamental genetic differences between bacteria and somatic cells, and this together with the inherent mathematical complexity of fluctuation analysis can lead to many pitfalls in its application. In addition there is considerable statistical error associated with the method. The use, misuse, and limitations of fluctuation analysis are reviewed here with the hope that such problems may be avoided.
1 Supported in part by USPHS Grants CA 39853 and CA 41525, the Alberta Heritage Foundation for Medical Research, and the R. E. "Bob" Smith Fund.
2 Fellow of the Alberta Heritage Foundation for Medical Research.
3 To whom requests for reprints should be addressed, at the Department of Cell Biology, Box 173, University of Texas M. D. Anderson Hospital and Tumor Institute at Houston, 1515 Holcombe Boulevard, Houston, TX 77030.
Received 4/29/87. Revised 10/22/87. Accepted 11/20/87.
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