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A New Childhood T-Cell Lymphoma Established in Nude Mice and in Vitro¹

Institute of Microbiology [R. A., C. J., G. B., M. G. M., T. M., G. F.] and Departments of Genetics [C. M., C. G.] and Pediatrics [L. C. M.], University of Turin and Department of Pathology, Regina Margherita Children's Hospital, 10126 Turin [M. F.]; and Department of Experimental Medicine, University of Rome, 00161 Rome [A. M.], Italy

A T-lymphoma cell line was established from a lymph node biopsy of a boy currently alive in complete remission. Neoplastic cells from this biopsy did not grow in vitro, whereas they formed a progressively growing s.c. tumor in splenectomized and sublethally irradiated nude mice and became serially transplantable in splenectomized and sublethally irradiated nude mice with a stable latency time. After the fourth transplant, cells were stored in liquid nitrogen and referred to as ST-4 cells. ST-4 cells display a membrane phenotype and a karyotype similar to that of the biopsy cells. After thawing, ST-4 cells grow both in splenectomized and sublethally irradiated nude mice and in vitro. They do not secrete interferon or interleukin 2, do not have natural killer activity, and do not respond to mitogen or alloantigen stimulation. The stable features of these T-lymphoma cells and the availability of normal autologous lymphocytes from the patient make this in vivo system quite unique and of importance for studies in tumor immunotherapy.

¹ This work was supported by grants from Ric. Finaliz. Reg. Piemonte, National Research Council, Italy (PF No. 86.00414.44), Comit. Piem. "G. Ghirotti," and Italian Cancer Research Association.

² To whom requests for reprints should be addressed, at Istituto di Microbiologia, Via Santena 9, 10126 Torino, Italy.

Received 8/14/87. Revised 11/23/87. Accepted 12/ 1/87.

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