This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hines, D. L. Search for Related Content PubMed PubMed Citation Articles by Hines, D. L.

Viral Oncogene Expression during Differentiation of Abelson Virus-infected Murine Promonocytic Leukemia Cells¹

Trudeau Institute, Saranac Lake, New York 12983

A cloned, virus-producing, tumorigenic, promonocytic leukemia cell line, AC8, derived from an Abelson murine leukemia virus-infected mouse can differentiate in vitro. Differentiated cells, purified from a population containing undifferentiated cells on the basis of expression of the macrophage differentiation antigens Mac-1 (C3 receptor) and F4/80, were phagocytic, produced lysozyme, were less tumorigenic, and had a reduced replicative capacity compared with undifferentiated cells. Differentiated cells produced less infectious Abelson virus than undifferentiated cells, but helper virus production was unaffected by differentiation. Cloning studies indicated that differentiation was the cause rather than the effect of reduced Abelson virus production. However, the intracellular amount and the tyrosine-specific protein kinase activity of the Abelson virus oncogene product, P120v-abl, were not affected by differentiation of the leukemic cells. Thus, these results show that Abelson virus-transformed myeloid lineage cells can differentiate without expression of the viral oncogene product being affected, which implies, in turn, that P120v-abl expression is not sufficient for maintaining transformation by blocking differentiation.

¹ This work was supported by Public Health Service Grant CA-40597 from the National Cancer Institute, and a grant from the J. M. Foundation.

Received 8/24/87. Revised 12/ 2/87. Accepted 12/28/87.