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Augmentation of Release of Cytotoxin from Murine Bone Marrow Macrophages by -Interferon

Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa 199-01, Japan

-Interferon (IFN-) was shown to amplify cytotoxin release from murine bone marrow-adherent macrophages in response to an animal lectin as a second stimulus. This stimulation of cytotoxin release was demonstrated both in vitro and in vivo and was maximal 2 h after treatment with IFN-. No change in expression of surface antigens was detected on bone marrow-adherent cells treated with IFN-. IFN- also augmented production of cytotoxin from bone marrow-adherent cells that had been cultured for several days. This cytotoxin was inhibited by rabbit anti-tumor necrosis factor serum and had a molecular weight of 70,000. These findings showed that both freshly isolated and cultured bone marrow macrophages could release cytotoxin and that cytotoxin production in response to a second stimulus was augmented by IFN-. The results also suggest that IFN- can activate all macrophages in vivo, irrespective of their states of maturation and differentiation.

¹ To whom requests for reprints should be addressed.

Received 8/10/87. Revised 12/ 5/87. Accepted 12/28/87.