This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Averbeck, D. Articles by Moustacchi, E. Search for Related Content PubMed PubMed Citation Articles by Averbeck, D. Articles by Moustacchi, E.

Repair of 4,5',8-Trimethylpsoralen plus Light-induced DNA Damage in Normal and Fanconi's Anemia Cell Lines¹

Institut Curie, Section de Biologie, 26 rue d'Ulm, 75231 Paris cédex 05, France

In conditions in which solely monoadducts (MA) are induced in DNA, i.e., treatment with 4,5',8-trimethylpsoralen and 405 nm radiation, Fanconi's anemia cells (FA) appear to be more sensitive than normal human fibroblasts (1 BR/3) to cytotoxicity. The repair of such induced MA is impaired in FA compared to normal cells. When increasing the proportion of DNA interstrand cross-links (CL) over MA using a reirradiation protocol, the differential sensitivity between FA and normal human cells increases. Moreover, for a constant number of total adducts or at different ratios of CL over MA, the repair of CL is systematically hampered in FA as compared to normal cells. Incision of CL being progressively diminished by increasing amounts of MA in normal cells (D. Papadopoulo, D. Averbeck, and E. Moustacchi, Photochem. Photobiol., 47: 321–326, 1988), we show here that it is even more so for FA cells.

¹ This work was supported by grants from CNRS, INSERM (Contract 852017), CEA (Saclay) France, and CEE Grant BIO-151 F.

Received 8/ 3/87. Revised 12/ 1/87. Accepted 12/22/87.

This article has been cited by other articles:

				D. Sridharan, M. Brown, W. C. Lambert, L. W. McMahon, and M. W. Lambert Nonerythroid {alpha}II spectrin is required for recruitment of FANCA and XPF to nuclear foci induced by DNA interstrand cross-links J. Cell Sci., March 1, 2003; 116(5): 823 - 835. [Abstract] [Full Text] [PDF]

				K. M. Vasquez, J. Christensen, L. Li, R. A. Finch, and P. M. Glazer Human XPA and RPA DNA repair proteins participate in specific recognition of triplex-induced helical distortions PNAS, April 30, 2002; 99(9): 5848 - 5853. [Abstract] [Full Text] [PDF]

				K. R. Kumaresan and M. W. Lambert Fanconi anemia, complementation group A, cells are defective in ability to produce incisions at sites of psoralen interstrand cross-links Carcinogenesis, April 1, 2000; 21(4): 741 - 751. [Abstract] [Full Text] [PDF]

				L. W. McMahon, C. E. Walsh, and M. W. Lambert Human alpha Spectrin II and the Fanconi Anemia Proteins FANCA and FANCC Interact to Form a Nuclear Complex J. Biol. Chem., November 12, 1999; 274(46): 32904 - 32908. [Abstract] [Full Text] [PDF]

				D. W. Brois, L. W. McMahon, N. I. Ramos, L. M. Anglin, C. E. Walsh, and M. W. Lambert A deficiency in a 230 kDa DNA repair protein in Fanconi anemia complementation group A cells is corrected by the FANCA cDNA Carcinogenesis, September 1, 1999; 20(9): 1845 - 1853. [Abstract] [Full Text] [PDF]

				K. M. Vasquez, J. Christensen, L. Li, R. A. Finch, and P. M. Glazer Human XPA and RPA DNA repair proteins participate in specific recognition of triplex-induced helical distortions PNAS, April 30, 2002; 99(9): 5848 - 5853. [Abstract] [Full Text] [PDF]