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Differential Induction by Interferons of Major Histocompatibility Complex-encoded and Non-Major Histocompatibility Complex-encoded Antigens in Human Breast and Ovarian Carcinoma Cell Lines¹

Departments of Medicine [C. M. B., S. E. N., L. F. W., D. S. L., R. C. B.], Community and Family Medicine and Biometry [D. V. D.], and Microbiology and Immunology [R. C. B.], Duke University Medical Center, Durham, North Carolina 27710 and The Cetus Corporation [D. R.], Emeryville, California 94608

Treatment of cancer cells with interferons can modulate expression of cell surface antigens, particularly those of the major histocompatibility complex (MHC). To examine the effect of recombinant - and -interferons on expression of non-MHC antigens, murine monoclonal antibodies have been used to quantitate 14 distinct tumor-associated cell surface antigens from five breast cancer cell lines and five ovarian cancer cell lines using a live cell radioimmunoassay. Both Class I and Class II MHC antigens could be augmented or induced with -interferon. Significantly increased expression of MHC antigens was observed in nine of 10 cell lines with induction indices as high as 11-fold. When 17 non-MHC epitopes were measured on 10 cell lines, minimal (1.3–2.7-fold) induction was observed in 10 of the 170 instances evaluated. Expression of only two epitopes, 2G3 and 735B11, was increased on more than one cell line. On six cell lines expression of non-MHC epitopes could not be increased. Consequently, among many different cell surface determinants, interferons produced a highly selective augmentation or induction of MHC antigens, whereas augmentation or induction of other tumor-associated antigens was apparently restricted to a few epitopes.

¹ Funded in part by Grant 5-RO1CA39930.

² To whom requests for reprints should be addressed, at Box 3843, Duke University Medical Center, Durham, North Carolina 27710.

Received 5/30/88. Revised 11/ 3/88. Revised 2/17/89. Accepted 2/28/89.

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