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Acquisition by the Murine Host of Responsiveness toward Various Neoplastic Cell Lines, but not toward Self, through Adoptive Transfer of a Helper T-Lymphocyte Clone with Antiself Specificity¹

Laboratory of Molecular Biology, State University of Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium

Self-antigens, when expressed on neoplastic cells, have been shown to exhibit a certain antigenicity. We attempted to apply this antigenicity to enhance antitumor immune responses. Cells from the syngeneic, CD4⁺, CD8^-, helper T-lymphocyte clone TE2 were adoptively transferred to C57BL/6 mice. TE2 lymphocytes recognize a self-antigen on splenocytes that is expressed aberrantly on the neoplastic cell lines EL4/8, EL4/13, B16-BL6, and PG19, all of C57BL/6 origin. Their adoptive transfer led to the rejection by the host of the former neoplastic cells and of 3LL carcinoma cells, administered 2 months later; inocula 40 to 80 times the minimal lethal size were rejected and conveyed to the mice a 10-fold enhanced cytotoxic T-lymphocyte response. Despite the autoimmune responsiveness of the TE2 T-lymphocytes, no graft-versus-host reaction was apparent. This conclusion is based on the absence of a polyclonal B-lymphocyte stimulation in the host, the stable number of residual donor TE2 cells, and the general health of the recipient mice. Consequently, the autoimmune and tumor-responsive TE2 cells, transplanted into the immune environment of the host, exhibit a specificity that is restricted toward neoplastic cells.

¹ Supported by a grant from the Algemene Spaar en Lijfrentekas, Brussels, Belgium.

² To whom requests for reprints should be addressed.

Received 1/18/89. Revised 4/13/89. Accepted 4/20/89.

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