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Growth-associated Shedding of a Tumor Antigen (CE7) Detected by a Monoclonal Antibody¹

Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Sapporo 060, Japan

A monoclonal antibody was developed against an antigen, termed CE7, which was highly expressed on the surface of rat fibrosarcoma KMT-17 cells (clone A3) cultured in low serum medium (A3-1% FCS). The CE7 antigen was not detectable on A3 cells cultured in ordinary high serum medium (A3–10% FCS), on in vivo passaged A3 cells, or on parental in vivo KMT-17 cell line. However, immunoelectron microscopy and Western blot analyses indicated that CE7 antigen was produced by these tumor cells in all circumstances but was shed from their surfaces in vesicular form into the surrounding tissue culture medium or ascites, unless low serum concentration prevailed and disappeared from their cell surfaces. We have previously reported that the immunogenicity of A3 cells was increased when the serum concentration was lowered from 10% to 1% and the phenomenon paralleled the CE7 antigen expression on the A3 cells. These results suggest that the CE7 antigen could be a tumor-associated rejection antigen and that the expression of the CE7 antigen on A3-1% FCS cells (which is shed by high serum culture or in vivo transplantation and disappears from the cell surface) may play a role in immunological responses against the tumor cells.

¹ This work was supported by a Grant-in-Aid for Cancer Research from the Japanese Ministry of Education, Science and Culture.

² Present address: National Cancer Institute, NCI-Navy Medical Oncology Branch, Naval Hospital Bethesda, Bldg. 8, Bethesda, MD 20814. To whom requests for reprints should be addressed.

Received 2/ 1/89. Revised 4/12/89. Accepted 4/19/89.

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