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Assignment of Common Allele Loss in Osteosarcoma to the Subregion 17p13¹

Radiation Biology Center [J. T., K. I., M. S. S., M. I., M. K.] and Department of Orthopaedic Surgery, Faculty of Medicine [J. T., M. S., Y. K., T. Y.], Kyoto University, Yoshida-konoecho, Sakyo-ku, Kyoto 606, Japan; and Howard Hughes Medical Institute and Department of Human Genetics, University of Utah Medical Center, Salt Lake City, Utah 84132 [Y. N.]

Human osteosarcomas frequently show loss of alleles on chromosome 17 as well as those on chromosome 13 that harbors the retinoblastoma gene, indicating concerted operation of another tumor-suppressing gene on chromosome 17. To assign the affected gene to a defined region of chromosome 17, we performed mitotic recombination/deletion mapping by the use of 10 polymorphic loci on chromosome 17. Of 37 tumors studied, 28 (75.7%) showed loss of heterozygosity on chromosome 17. The affected regions varied among tumors, ranging in extent from a whole chromosome to a distal segment of the short arm. However, allele loss in one region, notably in 17p13 between D17S1 and D17S30, was common to all 28 tumors, suggesting the presence of a tumor-suppressing gene in this defined region.

¹ Supported in part by grants from the Ministry of Education, Science, and Culture, and the Ministry of Health and Welfare, Japan.

² Present address: Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, 243 Charles Street, Boston, MA 02114.

³ To whom requests for reprints should be addressed.

Received 4/24/89. Revised 8/11/89. Accepted 8/18/89.