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Expression of Transforming Growth Factor in Normal Human Adult Kidney and Enhanced Expression of Transforming Growth Factors and ß1 in Renal Cell Carcinoma

Surgery Branch, National Cancer Institute, NIH, Bethesda, Maryland 20892

Normal kidney and renal cell carcinoma tissues from ten patients were studied for mRNA and DNA for both transforming growth factors and ß1. Northern and Southern hybridizations were conducted on samples extracted from the solid tumor and surrounding normal tissues and two tumor-derived cell lines. Low levels of constitutive expression of TGF- mRNA were detected in all normal kidney tissues; six of the ten patients, however, demonstrated an increased (2- to 8-fold) expression of TGF- in the tumor versus normal kidney as determined by densitometry of RNA blots. All ten patients had elevated mRNA levels for TGF-ß1 in the tumor (2.5- to 22-fold increase) relative to normal kidney. Two tumor-derived cell lines also expressed TGF- and TGF-ß1 mRNA. Southern blot hybridization of the DNA extracted from the normal tumor pairs revealed no gene amplification or gross rearrangement for either the TGF- or TGF-ß1 genes. These results demonstrate the expected constitutive expression of TGF-ß1 by normal kidney; however, the constitutive expression of TGF- by Northern blot analysis in normal adult human kidney is previously unreported. Enhanced expression of TGF- and TGF-ß1 mRNA in solid tumor may be related to the development of renal cell carcinoma.

¹ Present address: Department of Urology, Jefferson Medical College, 1025 Walnut Street, Philadelphia, PA 19107. To whom requests for reprints should be addressed.

Received 4/ 3/89. Revised 9/18/89. Accepted 9/21/89.

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