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Expression and Precursor Processing of Neuropeptide Y in Human and Murine Neuroblastoma and Pheochromocytoma Cell Lines¹

Laboratory of Molecular Endocrinology, University Department of Clinical Chemistry, Rigshospitalet 6321, Blegdamsvej 9, DK-2100 Copenhagen, Denmark

The synthesis and processing of the precursor for neuropeptide Y (NPY) were studied in 16 human and murine neuroendocrine cell lines. Eight of the cell lines, NS-20Y, PC12, LA-N-5, CHP-234, SMS-KCNR, SH-SY5Y, SMS-KCN, and BE(2)-M17, produced sufficient quantities to permit chromatographic characterization of the NPY immunoreactivity. Although the cell lines varied in the amount of NPY they produced, both within and between cell lines, they displayed a relatively constant pattern of posttranslational modifications. In contrast to observations in tumor extracts (M. M. T. O'Hare and T. W. Schwartz. Cancer Res., 49: 7010–7014, 1989), all cell lines studied contained a substantial amount of the intracellular NPY in the form of the unprocessed propeptide, 57% (range, 33–72%) as characterized by both gel filtrations (32 experiments in 8 cell lines) and "in vitro conversion" with endoproteinase Lys-C. In the majority, 4 of 6 cell lines studied, almost all of the NPY, which by size corresponded to the mature 36-amino acid form, was amidated as assessed by isoelectric focusing and by a radioimmunoassay specific for the COOH-terminal amide group of the peptide. Both the propeptide and smaller molecular forms of NPY were secreted from the cell cultures; however, proteolytic degradation in the tissue culture medium prevented a detailed, meaningful characterization of these peptides. It is concluded that many neuroendocrine cell lines, especially those derived from human neuroblastomas, express the NPY gene; the cells display a partly impaired dibasic processing capacity but they generally amidate the products efficiently.

¹ This investigation was supported in part by the Danish Cancer Association, and the Danish Medical Research Council. T. W. S. was the recipient of a Research Professorship in Molecular Endocrinology from the Danish Medical Research Council and the Weimann Foundation.

² To whom requests for reprints should be addressed.

Received 5/25/89. Revised 9/11/89. Accepted 9/21/89.