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Department of Radiobiology [S. K., N. N., M. H., M. A.] and Department of Genetics [A. A. A.], Radiation Effects Research Foundation, Hiroshima 732, Japan; Pacific Northwest Research Foundation, Seattle, Washington 98101 [M. A. B.]; and Biomedical Sciences Division, Lawrence Livermore National Laboratory, University of California, Livermore, California 94550 [R. H. J.]
A modified method was developed for measuring the frequency of variant erythrocytes at the glycophorin A locus using a single beam cell sorter (SBS). Fluorescein- or phycoerythrin-labeled monoclonal antibodies specific for the M or N glycophorin A alleles were used for the SBS assay. To prevent contamination of nucleated cells in the sorting windows, the nucleated cells in the fixed erythrocyte sample were stained with propidium iodide before flow sorting. Blood samples were obtained from atomic bomb survivors who were heterozygous for the MN blood type, and the frequencies of the hemizygous and homozygous variant of the M or N glycophorin A allele were measured by the SBS. For the three types of variants, hemizygotes for M and N allele (Nø and Mø) and homozygotes for M allele (MM), the variant frequency measured by the SBS correlated well with that previously determined by a dual beam cell sorter. Variant frequencies of the Nø, Mø, and MM cell types in atomic bomb survivors determined by SBS measurements were found to increase with radiation dose (DS86, kerma) as well as with the frequency of chromosome aberrations in lymphocytes.
1 The Radiation Effects Research Foundation (formerly ABCC), which sponsored this project, was established in April 1975 as a private nonprofit Japanese Foundation, supported equally by the Government of Japan through the Ministry of Health and Welfare and by the Government of the United States through the National Academy of Sciences under contract with the Department of Energy.
2 To whom requests for reprints should be addressed, at Department of Radiation Biology, Radiation Effects Research Foundation 5-2, Hijiyama Park, Minami-ku, Hiroshima 732, Japan.
Received 6/13/88. Revised 10/19/88. Accepted 10/24/88.
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