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Induction by the Guanosine Analogue Oxanosine of Reversion toward the Normal Phenotype of K-ras-transformed Rat Kidney Cells¹

Institute of Microbial Chemistry, 3-14-23 Kamiosaki, Shinagawa-ku, Tokyo 141 [O. I., S. A., K. U., T. T.], and Showa College of Pharmaceutical Science, Tsurumaki 5-1-8, Setagaya-ku, Tokyo 154 [S. K., M. H.], Japan

Oxanosine, a guanosine analogue antibiotic, altered the "transformed" morphology of rat kidney cells integrating a temperature-sensitive K-ras gene into "normal" morphology at 34°C, a permissive temperature. Oxanosine also increased the cellular content of fibronectin to the normal level. The cells under these conditions had lower levels of guanine nucleotides and unstable and less palmitylated M_r 21,000 protein (K-ras gene product, referred to as p21). These changes in p21 were the same as those which occurred to p21 in the cells cultured in the absence of oxanosine at 39.5°C, a nonpermissive temperature.

¹ Supported in part by Grants-in-Aid from the Ministry of Education, Science and Culture and from the Ministry of Health and Welfare, Japan.

² To whom requests for reprints should be addressed.

Received 2/ 3/88. Revised 7/ 8/88. Revised 11/10/88. Accepted 11/15/88.

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