This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shirakawa, F. Articles by Yamashita, U. Search for Related Content PubMed PubMed Citation Articles by Shirakawa, F. Articles by Yamashita, U.

Autocrine Stimulation of Interleukin 1 in the Growth of Adult Human T-Cell Leukemia Cells¹

First Department of Internal Medicine [F. S., Y. T., S. O., S. E.] and Department of Immunology [U. Y.], University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, Yahatanishiku, Kitakyushu 807, Japan

In a previous study, we reported that adult T-cell leukemia (ATL) cells produce interleukin 1 (IL1)-like factors that stimulate murine thymocyte proliferation, the production of interleukin 2 (IL2), and the expression of IL2 receptors (IL2R) on normal human T-cells in the presence of concanavalin A. In this communication, we studied the effect of IL1 on the growth of ATL cells in vitro. When ATL cells freshly obtained from patients were cultured with recombinant (r) human IL1, IL1ß, or IL1-like factors produced by ATL cell lines, the growth of ATL cells was stimulated in a concentration-dependent manner. Maximum stimulation was observed at a concentration of 50–100 units/ml of IL1. The expression of IL2R on ATL cells was also enhanced by IL1, but the production of IL2 was not induced. These effects of rIL1 or ß were specifically inhibited by anti-IL1 or anti-IL1ß antibody. Furthermore, the spontaneous growth of ATL cells was also inhibited by anti-IL1 antibody, but not by anti-IL1ß antibody. ATL cells exhibited enhanced expression of IL1 receptors on their surface as detected by the binding of ¹²⁵I-labeled rIL1. These results suggest that IL1 produced by ATL cells stimulates the growth of ATL cells by an autocrine mechanism.

¹ This work was supported in part by Grants-in-Aid for: Scientific Research, from the Ministry of Education, Science and Culture, Japan (61480185); Cancer Research, from the Ministry of Health and Welfare, Japan (60-29); and Cancer Research, from Fukuoka Cancer Association.

² To whom requests for reprints should be addressed.

Received 8/ 2/88. Revised 11/15/88. Accepted 11/28/88.

This article has been cited by other articles:

				K. Enya, H. Hayashi, T. Takii, N. Ohoka, S. Kanata, T. Okamoto, and K. Onozaki The interaction with Sp1 and reduction in the activity of histone deacetylase 1 are critical for the constitutive gene expression of IL-1{alpha} in human melanoma cells J. Leukoc. Biol., January 1, 2008; 83(1): 190 - 199. [Abstract] [Full Text] [PDF]

				D S Hallegua and M H Weisman Potential therapeutic uses of interleukin 1 receptor antagonists in human diseases Ann Rheum Dis, November 1, 2002; 61(11): 960 - 967. [Abstract] [Full Text] [PDF]

				Y. Tanaka, S. Mine, C. G. Figdor, A. Wake, H. Hirano, J. Tsukada, M. Aso, K. Fujii, K. Saito, Y. van Kooyk, et al. Constitutive Chemokine Production Results in Activation of Leukocyte Function-Associated Antigen-1 on Adult T-Cell Leukemia Cells Blood, May 15, 1998; 91(10): 3909 - 3919. [Abstract] [Full Text] [PDF]