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Presence of a Constitutive Paracrine ß-Interferon in v-mos-bearing Nonmalignant Reverted Cells¹

Unité 43 INSERM, Hôpital St. Vincent de Paul, 74 avenue Denfert-Rochereau, 75674 Paris Cedex 14, France

A stable nonmalignant revertant cell line was derived from Moloney murine sarcoma virus-transformed BALB/c cells after long-term cultivation in the presence of murine type I interferon (IFN). These cells gradually established resistance to exogenous IFN and were also seen to contain IFN-dependent proteins. The presence of an endogenous IFN was confirmed by the results of Northern blot analysis and in situ hybridization with an IFN-ß probe, showing that only mRNA specific for IFN-ß- could be found in the uninduced reverted cells. The latter synthesized only a small amount of IFN-ß protein and exhibited few IFN-specific membrane receptors, which bound recombinant IFN-ß with a high affinity. After treatment with IFN antibody, the overexpression of H-2 major histocompatibility antigen genes was significantly down-regulated. These findings strongly suggest the existence in this reverted cell line of a constitutive IFN which, acting through an autocrine and/or paracrine mechanism, might play a role in maintaining the reverted state.

¹ This investigation was supported by the Ligue Nationale Française Contre le Cancer, Comité de Paris.

² To whom requests for reprints should be addressed.

Received 5/24/88. Revised 11/15/88. Accepted 11/23/88.

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