This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Srivastava, P. K. Articles by Old, L. J. Search for Related Content PubMed PubMed Citation Articles by Srivastava, P. K. Articles by Old, L. J.

Identification of a Human Homologue of the Murine Tumor Rejection Antigen GP96¹

Samuel Freeman Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York 10021

A family of cell surface glycoproteins with a molecular weight of 96,000 (gp96) has recently been implicated in the individually distinct immunogenicity of chemically induced sarcomas of inbred mice. Rabbit antiserum to murine gp96 detects an antigenically related M_r 96,000 cell surface glycoprotein on two cultured human melanoma cell lines, SK-MEL-13 and SK-MEL-177. Molecular probes for 5' and 3' ends of the murine gp96 gene detect a 3.5-kilobase transcript in RNA preparations from melanoma cells, similar to the murine gp96 transcript. While 5' probes do not hybridize to Southern blots of genomic human DNA, the 3' probes identify several distinct bands under stringent hybridization and washing conditions. This suggests that the 3' end of the gp96 gene is more conserved than its 5' end. No gross alterations in gp96 gene organization were detected in melanoma cells. B-lymphoblastoid cell lines derived from four different individuals also showed no restriction fragment polymorphism in the gp96 gene.

¹ This work was supported by a program project Grant CA-08748 of the National Cancer Institute, a grant from the Samuel Freeman Trust, and an American Cancer Society in-house award (114J) to P. K. S.

² Recipient of a First Independent Research Support and Transition Award of the National Cancer Institute (CA-44786). To whom requests for reprints should be addressed at Box 1215, Department of Pharmacology, Mount Sinai School of Medicine, City University of New York, New York, NY 10029.

Received 9/19/88. Revised 12/ 5/88. Accepted 12/14/88.

This article has been cited by other articles:

				M. W. Graner and D. D. Bigner Chaperone proteins and brain tumors: Potential targets and possible therapeutics Neuro-oncol, July 1, 2005; 7(3): 260 - 278. [Abstract] [PDF]