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Slowing of Cell Cycle Traverse for Cells in Exponential Monolayer Cultures Placed into Plateau-fed and Starved Medium¹

Department of Surgery and Oncology, Johns Hopkins Medical School, and Baltimore Veterans Administration Medical Center, Baltimore, Maryland 21205 [D. C. A., K. K. B.]; Department of Surgery, The University of New Mexico, and The Albuquerque Veterans Administration Medical Center, Albuquerque, New Mexico 87108 [S. A., S. C.]; and Robertson Electronics, Albuquerque, New Mexico 87123 [J. R.]

MCa-11 tumor cells in exponential monolayer cultures were pulse/chase-labeled with [³H]thymidine and then regrown in fresh, plateau-fed, or starved medium. We measured the DNA content and autoradiographic labeling of these cells by absorption cytophotometry at intervals of 0, 2, 4, 8, 12, and 24 h to follow the progress through the cell cycle of those cells which had incorporated isotope. We found that for the cells grown in plateau-fed and starved medium the G₀/G₁, S, and G₂ phases of the cell cycle were prolonged when compared to those for cells grown in fresh medium. These results show that, under adverse microenvironmental conditions, the growth of tumor cells can be regulated in all phases of the cell cycle, and that this regulation can include lengthening and even cessation of replicative DNA synthesis.

¹ Supported by the Veterans Administration. This the second in a series of papers from this laboratory comparing flow and absorption DNA measurements of the same cells.

² To whom request for reprints should be addressed, at The Department of Surgery, Dispensary 681, Johns Hopkins Hospital, 600 North Wolfe St., Baltimore, MD 21205.

Received 6/ 6/88. Revised 9/15/88. Revised 11/18/88. Accepted 12/13/88.

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