This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hollenberg, S. M. Articles by Evans, R. M. Search for Related Content PubMed PubMed Citation Articles by Hollenberg, S. M. Articles by Evans, R. M.

Identification of Two Regions of the Human Glucocorticoid Receptor Hormone Binding Domain That Block Activation¹

Department of Biology, University of California at San Diego, La Jolla 92093 [S. M. H.], and Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla [S. M. H., V. G., R. M. E.], California 92138

The steroid-binding domain of the human glucocorticoid receptor has a dominant, negative influence over the activity of the remainder of the molecule. We have determined sequences within this domain that are responsible for this inhibition by creating truncations and internal deletions which remove part or all of the ligand-binding domain. Two apparently unrelated sequences within this region are shown to block the ability to stimulate transcription when fused to the carboxyl terminus of a functional, hormone-independent receptor derivative. These results support the possibility of two distinct protein-binding sites within the steroid-binding domain.