This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Shiraishi, Y. Articles by Sandberg, A. A. Search for Related Content PubMed Articles by Shiraishi, Y. Articles by Sandberg, A. A.

Malignant Lymphoma Antigen Expressed in Nude Mouse Tumor Cells Derived from Carcinogen-transformed Bloom's Syndrome B-Lymphoblastoid Cell Lines¹

Departments of Anatomy [Y. S.], Pathology [T. M., H. H.], and Surgery [K. A.], Kochi Medical School, Nankoku-City, Kochi 781-51, Japan; Department of Hematology, Kyushu Cancer Center, Fukuoka, Japan [M. K.]; and The Cancer Center, Southwest Biomedical Research Institute, Scottsdale, Arizona 85251 [A. A. S.]

From nude mouse tumors, in which malignantly transformed Bloom's syndrome (BS) B-lymphoblastoid cell lines were successfully transplanted into s.c. tissues, we have detected strong expression of malignant lymphoma (ML)-associated antigen on the cell surface, by using diluted sera of ML patients and indirect immunofluorescence. Even though carcinogen-treated BS B-lymphoblastoid cell lines expressed various types of cancer antigens (ML, ovarian cancer, stomach cancer, lung cancer, liver cancer, etc.) on the cell membrane as a mixed population (Y. Shiraishi and H. Soma, Proc. Natl. Acad. Sci. USA, 85: 8211–8215, 1988), the finding that BS malignant cells originating from nude mouse tumors expressed specific ML-associated antigen seemed significant for ML diagnosis. BS nude mouse tumors were successively transplantable from nude mice to nude mice (100%). Histopathological studies using an electron microscope demonstrated that most tumor cells in s.c. tissues of nude mice were lymphoid malignant cells. Gel electrophoresis and Western blotting analyses demonstrated that the antigen which characterized ML was a single band (M_r 97,000) and did not cross-react with the sera of other cancer patients or with normal sera. Chromosome analysis showed that the cell clones with ML-associated antigen had marker chromosomes involving t(6;?)(p25;?),t(9;?)(q34;?), del(10)(p13),t(12;14)(q24;q11). The expression of ML-associated antigen was also discussed in relation to the marker chromosomes.

¹ This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture, Japan.

² To whom requests for reprints should be addressed.

Received 6/19/89. Revised 12/27/89.