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Radiumhemmet, Karolinska Hospital [U. L., S. L., U. N., G. W.], Department of Histology, Karolinska Institute [U. L., S. L], and Laboratory of Human Genetics, The New York Blood Center, New York, New York [J. G.]
Bloom's syndrome (BS) cells display a characteristic genomic instability, notably an elevated frequency of sister-chromatid exchange. Replicating DNA in cultured BS cells was labeled with [3H]thymidine using several time schedules. Separation of DNA in agarose gels showed high molecular weight DNA and three classes of DNA replication intermediates: 20-kilobase DNA, 10-kilobase DNA, and Okazaki fragments. In contrast newly replicated DNA from normal cells showed no 20-kilobase DNA replication intermediates. Certain BS cells, exceptional in that their characteristic genomic instability has for unknown reasons been corrected, also differed from normal cells in having the 20-kilobase intermediate, but they differed from both normal cells and the other (the uncorrected) BS cells in lacking the 10-kilobase DNA replication intermediates.
1 This work was supported by grants from the King Gustaf V:s Jubilee Foundation, Karolinska Institute, and research grant HD04134 from the NIH.
2 To whom requests for reprints should be addressed, at Radiumhemmet, Karolinska Hospital, PO Box 60500, S-104 01 Stockholm, Sweden.
Received 7/28/89.
Revised 1/ 4/90.
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