| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Department of Pathology and Laboratory Medicine [E. J. B., R. J. M.] and Department of Radiation Oncology [E. N. H.], University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
In a previous study we described a correlation between the metastatic potential of transformed rat embryo cells (REC) and their release of type IV collagenolytic activity (S. Garbisa et al., Cancer Res., 47: 1523–1528, 1987). In the present study, we have identified a Mr 92,000 gelatinase released exclusively by metastatic cell lines in vitro; seven of eight highly metastic REC lines transformed by H-ras or H-ras plus v-myc released this enzyme. In contrast, the Mr 92,000 gelatinase was not detected in two separate nontumorigenic REC lines immortalized with H-ras or c-myc or in four independent tumorigenic REC lines (transformed by H-ras plus adenovirus E1A) with markedly reduced metastatic potential. Study of the Mr 92,000 gelatinase in tumor explants showed that its expression may be modulated in vivo. Not only was Mr 92,000 release enhanced in tumor explants from cell lines which released it in vitro, but its expression was evident in three primary tumors and six metastatic tumors from the one metastatic cell line that failed to release it in vitro. Explants from the nonmetastatic cell lines grown as tumors showed no Mr 92,000 gelatinase release. The heterogeneous expression of a number of other gelatinases with molecular weights of 52,000, 56,000 62,000, 68,000, 80,000, and 240,000 was observed, but no correlation with metastatic potential was apparent. The Mr 92,000 gelatinase had the characteristics of a secreted neutral metalloproteinase. It may be responsible for the type IV collagenolytic activity reported previously in conditioned medium from metastatic transformed REC and could in part be responsible for the differences in metastatic potential in these cell lines.
1 This work has been supported by Grant CA 46830 from the NIH to R. J. M. E. B. has been supported by T32CA09140; R. J. M. is the recipient of a Junior Faculty Research Award and E. N. H., a Clinical Oncology Career Development Award from the American Cancer Society.
2 To whom requests for reprints should be addressed, at Department of Radiation Oncology, University of Pennsylvania School of Medicine, 3400 Spruce Street, Philadelphia, PA 19104-4283.
Received 12/14/89.
Revised 2/28/90.
This article has been cited by other articles:
|
|
 |
|
  J. M. Lamar, K. M. Pumiglia, and C. M. DiPersio An Immortalization-Dependent Switch in Integrin Function Up-regulates MMP-9 to Enhance Tumor Cell Invasion Cancer Res., September 15, 2008; 68(18): 7371 - 7379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. Wang, X. Zhang, D. Qian, H. Y. Lin, Q. L. Li, D. L. Liu, G. Y. Liu, X. D. Yu, and C. Zhu Effect of Ubiquitin-Proteasome Pathway on Mouse Blastocyst Implantation and Expression of Matrix Metalloproteinases-2 and -9 Biol Reprod, February 1, 2004; 70(2): 481 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-S. Kim, E.-J. Lee, H.-R. C. Kim, and A. Moon p38 Kinase Is a Key Signaling Molecule for H-Ras-induced Cell Motility and Invasive Phenotype in Human Breast Epithelial Cells Cancer Res., September 1, 2003; 63(17): 5454 - 5461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. C. Durkan, J. E. Nutt, C. Marsh, P. H. Rajjayabun, M. C. Robinson, D. E. Neal, J. Lunec, and J. K. Mellon Alteration in Urinary Matrix Metalloproteinase-9 to Tissue Inhibitor of Metalloproteinase-1 Ratio Predicts Recurrence in Nonmuscle-invasive Bladder Cancer Clin. Cancer Res., July 1, 2003; 9(7): 2576 - 2582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Yan, H. Wang, Y. Toh, and D. D. Boyd Repression of 92-kDa Type IV Collagenase Expression by MTA1 Is Mediated through Direct Interactions with the Promoter via a Mechanism, Which Is Both Dependent on and Independent of Histone Deacetylation J. Biol. Chem., January 17, 2003; 278(4): 2309 - 2316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sanceau, D. D. Boyd, M. Seiki, and B. Bauvois Interferons Inhibit Tumor Necrosis Factor-alpha -mediated Matrix Metalloproteinase-9 Activation via Interferon Regulatory Factor-1 Binding Competition with NF-kappa B J. Biol. Chem., September 13, 2002; 277(38): 35766 - 35775. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Farina, M.-P. Masciulli, A. Tacconelli, L. Cappabianca, G. De Santis, A. Gulino, and A. R. Mackay All-trans-Retinoic Acid Induces Nuclear Factor {kappa}B Activation and Matrix Metalloproteinase-9 Expression and Enhances Basement Membrane Invasivity of Differentiation-resistant Human SK-N-BE 9N Neuroblastoma Cells Cell Growth Differ., August 1, 2002; 13(8): 343 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tsukada and T. Pourmotabbed Unexpected Crucial Role of Residue 272 in Substrate Specificity of Fibroblast Collagenase J. Biol. Chem., July 19, 2002; 277(30): 27378 - 27384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jiang and R. J. Muschel Regulation of Matrix Metalloproteinase-9 (MMP-9) by Translational Efficiency in Murine Prostate Carcinoma Cells Cancer Res., March 1, 2002; 62(6): 1910 - 1914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Durkan, J. E. Nutt, P. H. Rajjayabun, D. E. Neal, J. Lunec, and J. K. Mellon Prognostic Significance of Matrix Metalloproteinase-1 and Tissue Inhibitor of Metalloproteinase-1 in Voided Urine Samples from Patients with Transitional Cell Carcinoma of the Bladder Clin. Cancer Res., November 1, 2001; 7(11): 3450 - 3456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Schmalfeldt, D. Prechtel, K. Harting, K. Spathe, S. Rutke, E. Konik, R. Fridman, U. Berger, M. Schmitt, W. Kuhn, et al. Increased Expression of Matrix Metalloproteinases (MMP)-2, MMP-9, and the Urokinase-Type Plasminogen Activator Is Associated with Progression from Benign to Advanced Ovarian Cancer Clin. Cancer Res., August 1, 2001; 7(8): 2396 - 2404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. W. Wong, A. Lee, L. Shientag, J. Yu, Y. Dong, G. Kao, A. B. Al-Mehdi, E. J. Bernhard, and R. J. Muschel Apoptosis: An Early Event in Metastatic Inefficiency Cancer Res., January 1, 2001; 61(1): 333 - 338. [Abstract] [Full Text]
|
|
|
|
|
|
M. P. V. Shekhar, J. Werdell, and L. Tait Interaction with Endothelial Cells Is a Prerequisite for Branching Ductal-Alveolar Morphogenesis and Hyperplasia of Preneoplastic Human Breast Epithelial Cells: Regulation by Estrogen Cancer Res., January 1, 2000; 60(2): 439 - 449. [Abstract] [Full Text]
|
|
|
|
|
|
A. R. Farina, A. Tacconelli, A. Vacca, M. Maroder, A. Gulino, and A. R. Mackay Transcriptional Up-Regulation of Matrix Metalloproteinase-9 Expression during Spontaneous Epithelial to Neuroblast Phenotype Conversion by SK-N-SH Neuroblastoma Cells, Involved in Enhanced Invasivity, Depends upon GT-Box and Nuclear Factor {{kappa}}B Elements Cell Growth Differ., May 1, 1999; 10(5): 353 - 367. [Abstract] [Full Text]
|
|
|
|
|
|
T. Iizasa, T. Fujisawa, M. Suzuki, S.-i. Motohashi, K. Yasufuku, T. Yasukawa, M. Baba, and M. Shiba Elevated Levels of Circulating Plasma Matrix Metalloproteinase 9 in Non-Small Cell Lung Cancer Patients Clin. Cancer Res., January 1, 1999; 5(1): 149 - 153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Barille, C. Akhoundi, M. Collette, M.-P. Mellerin, M.-J. Rapp, J.-L. Harousseau, R. Bataille, and M. Amiot Metalloproteinases in Multiple Myeloma: Production of Matrix Metalloproteinase-9 (MMP-9), Activation of proMMP-2, and Induction of MMP-1 by Myeloma Cells Blood, August 15, 1997; 90(4): 1649 - 1655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Polette, C. Gilles, V. Marchand, M. Lorenzato, B. Toole, J.-M. Tournier, S. Zucker, and P. Birembaut Tumor Collagenase Stimulatory Factor (TCSF) Expression and Localization in Human Lung and Breast Cancers J. Histochem. Cytochem., May 1, 1997; 45(5): 703 - 710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Bu and T. Pourmotabbed Mechanism of Ca2+-dependent Activity of Human Neutrophil Gelatinase B J. Biol. Chem., June 14, 1996; 271(24): 14308 - 14315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Gum, E. Lengyel, J. Juarez, J. H. Chen, H. Sato, M. Seiki, and D. Boyd Stimulation of 92-kDa Gelatinase B Promoter Activity by ras Is Mitogen-activated Protein Kinase Kinase 1-independent and Requires Multiple Transcription Factor Binding Sites Including Closely Spaced PEA3/ets and AP-1 Sequences J. Biol. Chem., May 3, 1996; 271(18): 10672 - 10680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Alexander, E. Hansell, O Behrendtsen, M. Flannery, N. Kishnani, S. Hawkes, and Z Werb Expression and function of matrix metalloproteinases and their inhibitors at the maternal-embryonic boundary during mouse embryo implantation Development, January 6, 1996; 122(6): 1723 - 1736. [Abstract] [PDF]
|
|
|
|
 |
|
 B. Himelstein, R Canete-Soler, E. Bernhard, and R. Muschel Induction of fibroblast 92 kDa gelatinase/type IV collagenase expression by direct contact with metastatic tumor cells J. Cell Sci., January 2, 1994; 107(2): 477 - 486. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Birkedal-Hansen, W.G.I. Moore, M.K. Bodden, L.J. Windsor, B. Birkedal-Hansen, A. DeCarlo, and J.A. Engler Matrix Metalloproteinases: A Review Critical Reviews in Oral Biology & Medicine, January 1, 1993; 4(2): 197 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. O'Farrell and T. Pourmotabbed Identification of Structural Elements Important for Matrix Metalloproteinase Type V Collagenolytic Activity as Revealed by Chimeric Enzymes. ROLE OF FIBRONECTIN-LIKE DOMAIN AND ACTIVE SITE OF GELATINASE B J. Biol. Chem., September 1, 2000; 275(36): 27964 - 27972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Yan, H. Wang, and D. D. Boyd KiSS-1 Represses 92-kDa Type IV Collagenase Expression by Down-regulating NF-kappa B Binding to the Promoter as a Consequence of Ikappa Balpha -induced Block of p65/p50 Nuclear Translocation J. Biol. Chem., January 5, 2001; 276(2): 1164 - 1172. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
