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Expression of a M_r 32,000 Laminin-binding Protein Messenger RNA in Human Colon Carcinoma Correlates with Disease Progression¹

Department of Surgery, New England Deaconess Hospital [K. M., T. S. R., G. D. S.], and Division of Cellular and Molecular Biology, Dana-Farber Cancer Institute [K. M., J. M. W., H. Y., L. B. C.], Harvard Medical School, Boston, Massachusetts 02215

Cell surface receptors for laminin may play an important role in tumor migration and metastasis. To evaluate laminin receptor/laminin-binding protein expression in human colon carcinoma, surgical specimens of primary colon cancers and liver metastases were examined by blot hybridization of total RNA with a complementary DNA clone which encodes a M_r 32,000 human laminin-binding protein. The mRNA level of the laminin-binding protein was higher in primary colon carcinoma than in adjacent normal colonic epithelium in 20 of 21 cases. In all 6 cases of colon cancer liver metastases, the laminin-binding protein mRNA level was more than 3-fold greater in tumor than in adjacent normal liver tissue. The tumor/normal ratio of this laminin-binding protein mRNA expression in primary colon cancer has significant correlation with Dukes' classification (P < 0.001). Our results suggest that mRNA expression of the laminin-binding protein may be a marker of human colorectal cancer progression and biological aggressiveness.

¹ Supported by a grant from the National Cancer Institute, PO1-CA 44704-02. Presented at the 42nd Annual Meeting of the Society of Surgical Oncology, San Francisco, May 24, 1989.

² To whom requests for reprints should be addressed, at Department of Surgery, New England Deaconess Hospital, 110 Francis St. #3A, Boston, MA 02215.

Received 10/24/89. Revised 2/12/90.

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