This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Perry, R. R. Articles by Pass, H. I. Search for Related Content PubMed PubMed Citation Articles by Perry, R. R. Articles by Pass, H. I.

Sensitivity of Different Human Lung Cancer Histologies to Photodynamic Therapy

Thoracic Oncology Section, Surgery Branch [R. R. P., W. M., S. E., H. I. P.], and the Radiation Oncology Branch [J. B. M., A. R.], National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892

The relative sensitivities of different cancer histologies in a single site to photodynamic therapy (PDT) are unknown and methods to predict PDT sensitivity have not been described. The in vitro response to PDT of six established human lung cancer cell lines and one normal lung fibroblast cell line was studied using the clonogenic assay. Dose-response curves were determined for cells incubated in 25 µg/ml of Photofrin II for 2 h, followed by exposure to 630-nm light to total energies of 0-3150 J/m². None of the cell lines were sensitive to sensitizer alone or light alone. Differences in inherent PDT sensitivities as evaluated by survival curve parameters n, D_o, and light dose to yield 1% survival were observed among the cell lines. No clear correlation was found when inherent PDT sensitivity was compared with sensitizer uptake; however, a general association was noted between PDT sensitivity and the plating efficiency of the cell line. These data illustrate that differences in inherent PDT exist for in vitro systems. Such differences may explain some of the failures seen in clinical PDT.

¹ To whom requests for reprints should be addressed, at Head, Thoracic Oncology Section, Surgery Branch, National Cancer Institute, NIH, Building 10, Room 2B07, Bethesda, MD 20892.

Received 6/20/89. Revised 2/19/90.

This article has been cited by other articles:

				D. I. Fielding, G. A. Buonaccorsi, A. J. MacRobert, A. M. Hanby, M. R. Hetzel, and S. G. Bown Fine-Needle Interstitial Photodynamic Therapy of the Lung Parenchyma: Photosensitizer Distribution and Morphologic Effects of Treatment Chest, February 1, 1999; 115(2): 502 - 510. [Abstract] [Full Text] [PDF]