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Molecular Nature of in Vivo Mutations in Human Cells at the Autosomal HLA-A Locus¹

Department of Haematology, Flinders Medical Centre, Bedford Park SA 5042 [A. A. M., S. A. G., D. R. T., A. K.], and the Tissue Typing Laboratory, Red Cross Blood Transfusion Service, Pirie Street, Adelaide SA 5000 [G. B.], Australia

The mutations present in vivo in normal human cells were studied at the HLA-A locus by isolating mutant lymphocytes using antibody-complement immunoselection and cloning at limiting dilution. The molecular basis for mutation in 127 mutant lymphocytes from 10 individuals was determined by studying a variety of polymorphic gene loci on both arms of chromosome 6. No change was detected in 78 mutants (61.4%), gene deletion was detected in 11 (8.7%), and mitotic recombination was detected in 38 (29.9%). Neither gene conversion nor chromosome loss was detected. These observations document the mechanisms responsible for gene loss in normal human cells in vivo, emphasize the importance of mitotic recombination, and indicate the similarity between mutational mechanisms in normal cells and in cancer cells.

¹ This study was supported by the National Health and Medical Research Council of Australia and the Anti-Cancer Foundation of the Universities of South Australia.

Received 1/11/90. Revised 4/13/90.

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