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Early Effects of Radiotherapy in Small Cell Lung Cancer Xenografts Monitored by ³¹P Magnetic Resonance Spectroscopy and Biochemical Analysis¹

Department of Oncology, Finsen Institute, Rigshospitalet, Blegdamsvej 9, Copenhagen 2100 [P. E. G. K.]; Department of Chemistry 5, H. C. Ørsted Institute, University of Copenhagen, Universitetsparken, Copenhagen 2200 [E. J. P.]; NMR Center, Panum Institute, University of Copenhagen, Blegdamsvej 3, Copenhagen 2200 [B. Q.]; and Institute of Pathological Anatomy, University of Copenhagen, Frederik d. V's vej 11, Copenhagen 2100 [P. E. G. K., F. E., M. S-T.], Denmark

³¹P magnetic resonance spectroscopy (³¹P MRS) and biochemical analysis of extracts were applied to study the metabolic response to X-irradiation of small cell lung cancer in nude mice. Two small cell lung cancer xenografts, CPH SCCL 54A and 54B, with different radiosensitivity, although derived from the same patient, were studied. A total of 126 individual tumors were examined. Following 5.0-Gy irradiation, a reversible increase in the ATP/P₁ ratio, reaching twice the pretreatment level within 2 wk, was observed with ³¹P MRS, while 20 Gy induced a reversible decrease in the ATP/P₁ ratio. The t of this decline was 2 to 3 h for 54A and about 6 h for the less radiosensitive 54B. The ³¹P MRS data were compared with biochemical analysis of tumors freeze-clamped and extracted at similar intervals after 20 Gy. It appeared that an acute reversible increase in P₁ concentration was the major cause of the ATP/P₁ decrease induced by 20 Gy. A linear correlation between ATP/P₁ estimated by ³¹P MRS and by analytical biochemistry was found. The ATP/P₁ ratio may be valuable for early assessment of radiosensitivity of small cell lung cancer tumors.

¹ Supported by Danish Medical Research Council Grants 12-6915 and 12-8146.

² To whom requests for reprints should be addressed.

Received 7/26/89. Revised 4/27/90.

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