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Paracrine Growth Stimulation of Androgen-responsive Shionogi Carcinoma 115 by Its Autonomous Subline (Chiba Subline 2)¹

Department of Urology, School of Medicine [Y. F., N. S., K. A., T. I., R. S., J. S.], and Laboratory of Biophysical Chemistry, Faculty of Pharmaceutical Sciences [N. S.], Chiba University, Chiba-shi, 280 (School of Medicine) and 260 (Faculty of Pharmaceutical Sciences), Japan

Shionogi Carcinoma 115 cells (SC 115 cells) and Chiba Subline 2 cells (CS 2 cells) are clones of an androgen-responsive mouse tumor cell line and its autonomous subline, respectively. Since it was reported that the growth of cells from SC 115 was regulated by an androgen-induced fibroblast growth factor (FGF)-like peptide (1), the present study was aimed at examining whether a similar growth factor was secreted by CS 2 cells in the absence of testosterone. Although SC 115 cells did not grow in the serum-free medium without androgens, SC 115 cells could proliferate in mixed culture with CS 2 cells, suggesting stimulation of SC 115 cells by CS 2 cells. It was shown that CS 2 cells secreted a growth factor without the influence of testosterone, and this factor promoted the growth of SC 115 and CS 2 cells, as well as that of BALB/3T3 cells. The factor was partially purified from serum-free conditioned medium obtained from cultures of CS 2 cells.

It showed an affinity for heparin, stability to heat and acid treatments, a decrease in activity when anti-basic FGF antibody was added to cultures, and an estimated molecular weight of approximately 50,000. Therefore, the factor seemed to have the nature of an FGF-like peptide. It was concluded that, in the absence of testosterone, CS 2 cells produced an FGF-like growth factor which controlled the growth of both CS 2 cells and parent SC 115 cells, in autocrine and paracrine manners, respectively.

¹ Supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture (01010011); from the Ministry of Health and Welfare (1–42); and for Scientific Research from the Ministry of Education, Science and Culture (62480333), Japan.

² To whom requests for reprints should be addressed.

Received 9/15/89. Revised 4/27/90.

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