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Regulation of Progesterone Receptor Gene Expression¹

Milano Molecular Pharmacology Laboratory, Institute of Pharmacological Sciences, University of Milan Piazza Durante II I-20131 Milan [E. V., A. M.]; Laboratory of Applied Immunology Roche Piazza Durante II I-20131 Milan [M. G. C.]; Department of Surgical Gynecologic Oncology, Istituto Nazionale dei Tumori of Milan, Via Venezian I I-20133 Milan [F. R., R. F.]; and Institute of Radiology, University of Ferrara, Corso Giovecca 203 I-44100 Ferrara [A. P.], Italy

The levels of progesterone receptor (PR) mRNA, PR protein, and [³H]R5020 binding activity were measured in parallel experiments conducted on a T47D subline expressing the estrogen receptor. A significant increase of PR mRNA levels could be detected within 6 h of exposure of the cells to estradiol (10^-3 M). The changes in mRNA, however, did not lead to any variation of PR protein levels of [³H]R5020 binding activity.

A parallel analysis of PR mRNA and [³H]R5020 binding was then performed in a series of tumor biopsies. In estrogen receptor-positive and PR-positive tissues a correlation among the two values was found. It is postulated that the above mentioned data could reflect the existence of a difference in the mechanisms controlling the numerous steps of the PR synthesis in the various hormone-responsive tissues. This variability could allow an organ-specific response to the cyclic changes of circulating hormone.

¹ This research is principally founded by the P. F. Biotecnologie of the Italian National Council of Research and by the Pharmaceutical Company Hoffmann-La Roche.

² Recipient of a fellowship from the Italian Association for Cancer Research.

³ To whom requests for reprints should be addressed.

Received 2/26/90. Revised 5/11/90.

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