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Identification of Ultraviolet-inducible Proteins That Bind to a TGACAACA Sequence in the Polyoma Virus Regulatory Region¹

Comprehensive Cancer Center and Institute of Cancer Research, Columbia University, New York, New York 10032

The exposure of mammalian cells to UV light or other DNA-damaging agents induces several responses which may provide cellular defense mechanisms and also play a role in carcinogenesis. Employing a 257-base pair DNA fragment from the polyoma virus that contains the origin of replication and regulatory region of this virus, we have identified a set of DNA-binding proteins that are induced in normal rat fibroblasts at 6–24 h after UV exposure. These proteins bind to a specific octamer sequence (TGACAACA) designated the "UV response element." Purification of these inducible proteins on a UV response element affinity column revealed a set of proteins, among which the major protein has a molecular weight of 40,000, which co-purify with c-fos but do not react with antibodies to c-jun-encoded proteins. These UV-induced proteins may, in concert with other cellular components, play a role in mediating specific cellular responses to DNA damage in mammalian cells.

¹ This work was supported by a grant from the National Cancer Institute (CA 02111) and an award from the Lucille Markey Charitable Trust (to I. B. W.).

² Present address: Molecular Carcinogenesis Program, Naylor Dana Institute. American Health Foundation. Valhalla. NY 10595.

Received 3/ 8/90. Revised 5/22/90.

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