This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yamashita, Y. Articles by Nakano, H. Search for Related Content PubMed PubMed Citation Articles by Yamashita, Y. Articles by Nakano, H.

Induction of Mammalian DNA Topoisomerase II Dependent DNA Cleavage by Antitumor Antibiotic Streptonigrin

Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahimachi, Machida, Tokyo 194, Japan

Streptonigrin, a nonintercalative antitumor antibiotic, induced mammalian topoisomerase II dependent DNA cleavage in vitro. The cleavage activity of streptonigrin was comparable to that of demethylepipodophyllotoxin ethylidene-ß-D-glucoside at a low concentration (10 µM) but one-third lower at a higher concentration (>250µM). Exposure of a reaction mixture containing streptonigrin, DNA, and topoisomerase II to an elevated temperature (65°C) resulted in substantial reduction in DNA cleavage, suggesting that the mechanism of the topoisomerase II dependent DNA cleavage induced by streptonigrin was through the formation of a cleavable complex previously reported for topoisomerase II poisons such as 4'-(9-acridinylamino) methanesulfon-m-anisidide and epipodophyllotoxins.

¹ To whom requests for reprints should be addressed.

Received 11/ 6/89. Accepted 6/13/90.

This article has been cited by other articles:

				N. Fujii, Y. Yamashita, T. Mizukami, and H. Nakano Correlation between the Formation of Cleavable Complex with Topoisomerase I and Growth-Inhibitory Activity for Saintopin-Type Antibiotics Mol. Pharmacol., February 1, 1997; 51(2): 269 - 276. [Abstract] [Full Text] [PDF]