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Antitumor Efficacy of Vaccinia Virus-modified Tumor Cell Vaccine¹

Department of Pathology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 [T. I., D. Q. W., K. N., S. K., T. K., N. W.], and Shionogi Research Laboratories, Shionogi Co., Ltd., Fukushima, Osaka 553 [M. M.], Japan

The antitumor efficacies of vaccinia virus-modified tumor cell vaccines were examined in murine syngeneic MH134 and X5563 tumor cells. UV-inactivated vaccinia virus was inoculated i.p. into C3H/HeN mice that had received whole body X-irradiation at 150 rads. After 3 weeks, the vaccines were administered i.p. 3 times at weekly intervals. One week after the last injection, mice were challenged i.p. with various doses of syngeneic MH134 or X5563 viable tumor cells. Four methods were used for preparing tumor cell vaccines: X-ray irradiation; fixation with paraformaldehyde for 1 h or 3 months; and purification of the membrane fraction. All four vaccines were effective, but the former two vaccines were the most effective. A mixture of the membrane fraction of untreated tumor cells and UV-inactivated vaccinia virus also had an antitumor effect. These results indicate that vaccine with the complete cell structure is the most effective. The membrane fraction of UV-inactivated vaccinia virus-absorbed tumor cells was also effective. UV-inactivated vaccinia virus can react with not only intact tumor cells but also the purified membrane fraction of tumor cells and augment antitumor activity.

¹ This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan and grants from Arima Memorial Foundation for Medical Research.

² To whom requests for reprints should be addressed.

Received 3/19/90. Accepted 7/31/90.