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Immunohistochemical Localization of Basic Fibroblast Growth Factor in Astrocytomas¹

Department of Pathology, Division of Neuropathology [D. Z., D. C. M.], Department of Pathology [D. E. B.], The Kaplan Cancer Center [D. C. M., D. B. R., D. E. B.], and Department of Cell Biology and the Raymond and Beverly Sackler Foundation Laboratory [Y. S., D. B. R.], New York University Medical Center, New York, New York 10016

Because of the prominent neovascularization observed in the growth of brain tumors, we studied the occurrence of basic fibroblast growth factor (bFGF), a potent angiogenic factor in astrocytomas, the most aggressive of which often have marked vascular hyperplasia. Using immunohistochemical methods, we examined 21 examples of such tumors, 7 glioblastomas multiforme, 7 anaplastic astrocytomas, and 7 low grade astrocytomas. Using polyclonal and affinity-purified rabbit antisera to human bFGF, we detected immunoreactive bFGF in all cases of glioblastoma multiforme. bFGF was present in both endothelial cells and neoplastic astrocytes. In 4 of 7 anaplastic astrocytomas, the tumor astrocytes had bFGF immunoreactivity and, in 5 of 7 cases, endothelial cells were also immunopositive. In glioblastomas multiforme and anaplastic astrocytomas, capillaries adjacent to tumor showed bFGF immunoreactivity, whereas capillaries distant from the tumors were not immunostained. In low grade astrocytomas, astrocytic cells were weakly immunoreactive in 2 of 7 cases, and in only 1 of the 7 cases capillaries were immunostained. In each grade, reactive astroglial cells showed variable bFGF immunoreactivity. The immunostaining was not seen with the flow-through fraction obtained after affinity purification of the bFGF antiserum with pure recombinant bFGF. These results suggest a possible role for bFGF in tumor growth and in angiogenesis in astrocytomas.

¹ This work was supported by grants from The Council for Tobacco Research (2466) (D. E. B.) and The American Cancer Society (BC554C) and The National Institute of Health (CA42229) (D. B. R.). D. E. B. is an Irma T. Hirschl-Monique Weill-Caulier trust awardee. This work was presented in part at the 79th annual meeting of the United States and Canadian Academy of Pathology, Boston, 1990 (80).

² To whom requests for reprints should be addressed, at Department of Pathology, New York University Medical Center, 550 First Avenue, New York, NY 10016.

³ Present address: Department of Pathology, Mount Sinai School of Medicine, Box 1194, 1 Gustave Levy Place, New York, NY 10029.

Received 5/ 3/90. Accepted 8/ 7/90.

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