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Laboratory of Molecular Pharmacology, National Cancer Institute, NIH, Bethesda, Maryland 20892 [A. J. F., M. A. P., M. C. H.], and Applied Genetics, Inc., Freeport, New York 11520 [D. B. Y.]
DNA probes prepared from human O6-methylguanine-DNA methyltransferase complementary DNA were hybridized to mRNA isolated from human liver and fifteen human tumor cell lines proficient (Mer+) or deficient (Mer-) in transferase activity. Liver and Mer+ cells contained levels of transferase-specific mRNA that correlated with their transferase activity levels, whereas Mer- cells contained undetectable amounts of transferase mRNA. The mRNA levels were not induced in human cells by treatments that induce other DNA damage-inducible genes. These results demonstrate that in human cells the transferase gene is constitutively expressed, that its expression is related to activity levels, and that in Mer- tumor cells the expression of the transferase gene is probably blocked at the level of mRNA production.
1 To whom requests for reprints should be addressed, at Laboratory of Molecular Pharmacology, National Cancer Institute, Building 37, Room 5C07, NIH, Bethesda, MD 20892.
Received 7/26/90. Accepted 9/18/90.
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