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Suppression of Dimethylhydrazine-induced Carcinogenesis in Mice by Dietary Addition of the Bowman-Birk Protease Inhibitor¹

Department of Cancer Biology, Harvard School of Public Health, Boston, Massachusetts 02115

In the present study the effect of feeding the soybean-derived Bowman-Birk protease inhibitor (BBI) on dimethylhydrazine (DHM)-induced gastrointestinal tract and liver carcinogenesis in mice was examined. In this investigation we found the addition of 0.5 or 0.1% semipurified BBI or 0.1% purified BBI to the diet of DMH-treated mice resulted in a statistically significant suppression of angiosarcomas and nodular hyperplasia of the liver and adenomatous tumors of the gastrointestinal tract. Autoclaved BBI or BBI which had its trypsin inhibitory domain specifically inactivated was found to be ineffective in suppressing the induction of these liver and gastrointestinal tract lesions. The results of this study also indicate that BBI, included as 0.5% of the diet or less, has the ability to suppress carcinogenesis with no observed adverse effects on the health of the mice.

¹ This investigation was supported by NIH Grants CA 38246, CA 46496, CA 45734, and ES-00002 and by NIH Training Grant CA 09078.

² Present address: Department of Radiology, Bowman Gray School of Medicine, 300 South Hawthorne Road, Winston-Salem, NC 27104. To whom requests for reprints should be addressed.

³ Present address: Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104.

⁴ Present address: Laboratory of Neoplastic Disease Mechanisms, Dana-Farber Cancer Institute, Boston, MA 02115.

⁵ Present address: Mallory Institute of Pathology Foundation, Boston University, Boston, MA 02118.

Received 10/28/88. Revised 4/28/89. Revised 8/ 1/89. Revised 10/17/89. Accepted 10/25/89.

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