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Expression of Mitochondrial Cytochrome c Oxidase in Human Colonic Cell Differentiation, Transformation, and Risk for Colonic Cancer¹

Montefiore and Albert Einstein Medical Centers, Department of Oncology, Bronx, New York 10467 [B. G. H., H. K. H., L. H. A.], and Sloan-Kettering Cancer Center, New York, New York 10021 [M. L.]

In a panel of eight cloned complementary DNA sequences whose level of expression characterize colon cells as transformed in vivo and in vitro, one which may also serve as a marker of risk in familial polyposis and familial colon cancer flat mucosa has been identified as mitochondrial cytochrome c oxidase subunit 3. Mean level of expression of cytochrome c oxidase subunit 3 decreases progressively in colon adenomas and carcinomas relative to normal mucosa in vivo, and returns to higher levels present in biopsies of normal mucosa when the HT29 human colonic adenocarcinoma cell line is induced to differentiate with sodium butyrate. Quantitation of cytochrome c oxidase subunit 3 DNA by dot blots indicated that these changes in expression were not associated with alterations in the number of mitochondrial genomes.

¹ Supported in part by Grants CA41372, CA40558, and P30-CA13330 from the National Cancer Institute, Grant SIG-7 from the American Cancer Society, and a grant from the Aaron Diamond Foundation.

² To whom requests for reprints should be addressed, at Department of Oncology, Montefiore and Albert Einstein Medical Centers, 111 East 210th St., Bronx, NY 10467.

Received 2/15/89. Revised 8/ 7/89. Revised 11/14/89. Accepted 11/15/89.

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