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Potentiation of Invasive Capacity of Rat Ascites Hepatoma Cells by Adriamycin¹

Department of Internal Medicine [F. I.], Clinical Research for Lung Cancer [T. H.], and Department of Tumor Biochemistry [M. M., K. S., H. A.], Research Institute, The Center for Adult Diseases, Osaka, Osaka 537, Japan

The effect of Adriamycin on the invasive capacity of rat ascites hepatoma cells, W1, was studied. The invasive capacity of W1 cells was estimated in vitro by counting the number of penetrated single tumor cells and tumor cell colonies formed from the penetrated cells underneath a cultured mesothelial cell monolayer (H. Akedo et al., Cancer Res., 46: 2416–2422, 1986). A considerable increment of the invasive capacity was observed when the tumor cells had been treated with 1.0 to 20.0 µM Adriamycin. This augmentation of invasive capacity of tumor cells was partially inhibited by 60 µM N-acetylcysteine, a scavenger of free radicals. On the other hand, 60 µM N-acetylcysteine did not impair the cytotoxicity of Adriamycin for W1 cells measured by an in vitro tetrazolium-based colorimetric assay for cytotoxicity.

¹ This work was supported in part by a Grant-in-Aid from the Ministry of Health and Welfare for a Comprehensive 10-Year Strategy of Cancer Control and a grant from Osaka Association for the Prevention of Adult Diseases.

² To whom requests for reprints should be addressed.

Received 8/22/89. Revised 12/ 4/89.

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