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Amplified and Rearranged bcl-2 Gene in Two Lymphoma Cell Lines, FL-218 and FL-318, Carrying a 14;18 Translocation¹

The First Division, Department of Internal Medicine, Faculty of Medicine, Kyoto University, Kyoto 606 [R. A., S. F., H. O., S. T., M. H., F. M., I. K., T. K.], and Department of Clinical Laboratory, Kyoto City Hospital, Kyoto 604 [O. N.], Japan

Two human B-cell lines carrying a 14;18 chromosome translocation [t(14;18)(q32;q21)], designated FL-218 and FL-318, were established from effusion cells of two Japanese patients manifesting the transformed histology of follicular lymphoma. The FL-218 and FL-318 cell lines were composed of cells in the hyperdiploid range, which had two and three or four 18q- chromosomes, respectively. These 18q- chromosomes were not distinguishable from an 18q- chromosome derived from t(14;18) since they exhibited the same banding pattern. Southern blot analysis revealed that in both cell lines, breakage of the bcl-2 gene occurred within the major breakpoint cluster region and the truncated gene juxtaposed to an immunoglobulin heavy chain gene locus. The autoradiographic intensity of the retained fragment each on 18q- chromosome was more enhanced than that of the translocated fragment on 14q+ chromosome. These findings suggest that the extra 18q- chromosome found in t(14;18)-positive cancer does not arise from de novo independent t(14;18) but from duplication of a preexisting 18q- chromosome.

¹ This work was supported by Grants-in-Aid from the Ministry of Education, Science, and Culture (63570572) and from the Ministry of Health and Welfare of Japan (61-2).

² To whom requests for reprints should be addressed, at The First Division, Department of Internal Medicine, Faculty of Medicine, Kyoto University, 54 Shogoin-kawaramachi, Sakyo-ku, Kyoto 606, Japan.

Received 6/27/89. Revised 12/11/89.

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