This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Noël, A. C. Articles by Foidart, J.-M. Search for Related Content PubMed PubMed Citation Articles by Noël, A. C. Articles by Foidart, J.-M.

Invasion of Reconstituted Basement Membrane Matrix Is Not Correlated to the Malignant Metastatic Cell Phenotype¹

Laboratories of Biology [A. C. N., A. C., H. P. E., J-M. F.], Experimental Dermatology [A. C. N., B. V. N., C. M. L.], Histology [L. S.], and Nephrology [J. F.], University of Liege, Liege, Belgium

Interactions between tumor cells and basement membranes represent a critical step in the progression of neoplasia and in the metastatic process. Reconstituted basement membrane matrix, matrigel, has been recently used with the aim of developing an in vitro assay of tumor cell invasiveness. We have extended these studies by comparing the invasiveness of a large series of normal and malignant epithelial and mesenchymal cells of human and animal origin cultured on matrigel. Normal cells (fibroblasts, glomerular mesangial cells, keratinocytes), human fibrosarcoma cells (HT1080), and reticular sarcoma cells (M5076) clearly established invasive capabilities in the matrix. However, all the other tested cell lines, malignant or virally transformed cells invasive in vivo (MCF7, T47D, SA52, SW613, MO₄, A431, BeWo), as well as normal nontransformed cells (MOH22) were incapable of penetration. The morphological features of matrigel invasion by normal fibroblasts and HT1080 cells are described at the light and electron microscope levels. The extent of degradation of a radiolabeled matrigel is minimal and similar in several cell lines reported to be noninvasive or invasive in vivo. Our data suggest that matrigel does not provide a universal model to correlate the invasiveness of cells in vivo and in vitro.

¹ This study was supported by grants from the "Fonds de la Recherche Scientifique Médicale Belge" (3.4512.85), from the "Fonds Cancérologique de la CGER," from the Research Fund of the Faculty of Medicine of the University of Liege, by a Biotechnology Action Programme (0-278-B) of the CEC, and from the "Association contre le Cancer" (Belgium).

² Supported by a grant of the "Commissariat Général aux Relations Internationales de la Communauté Française de Belgique."

³ To whom requests for reprints should be addressed, at Laboratory of Biology, University of Liege, Tower of Pathology (B23), Sart Tilman, B 4000 Liege, Belgium.

Received 1/30/90. Accepted 10/22/90.

This article has been cited by other articles:

				Z.-Z. Zeng, Y. Jia, N. J. Hahn, S. M. Markwart, K. F. Rockwood, and D. L. Livant Role of Focal Adhesion Kinase and Phosphatidylinositol 3'-Kinase in Integrin Fibronectin Receptor-Mediated, Matrix Metalloproteinase-1-Dependent Invasion by Metastatic Prostate Cancer Cells Cancer Res., August 15, 2006; 66(16): 8091 - 8099. [Abstract] [Full Text] [PDF]

				Y. Jia, Z.-Z. Zeng, S. M. Markwart, K. F. Rockwood, K. M. W. Ignatoski, S. P. Ethier, and D. L. Livant Integrin Fibronectin Receptors in Matrix Metalloproteinase-1-Dependent Invasion by Breast Cancer and Mammary Epithelial Cells Cancer Res., December 1, 2004; 64(23): 8674 - 8681. [Abstract] [Full Text] [PDF]

				E. Maquoi, N. E. Sounni, L. Devy, F. Olivier, F. Frankenne, H.-W. Krell, F. Grams, J.-M. Foidart, and A. Noel Anti-Invasive, Antitumoral, and Antiangiogenic Efficacy of a Pyrimidine-2,4,6-trione Derivative, an Orally Active and Selective Matrix Metalloproteinases Inhibitor Clin. Cancer Res., June 15, 2004; 10(12): 4038 - 4047. [Abstract] [Full Text] [PDF]

				H. Zhang, G. Kelly, C. Zerillo, D. M. Jaworski, and S. Hockfield Expression of a Cleaved Brain-Specific Extracellular Matrix Protein Mediates Glioma Cell Invasion In Vivo J. Neurosci., April 1, 1998; 18(7): 2370 - 2376. [Abstract] [Full Text] [PDF]