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Absence of Retinoblastoma Protein Expression in Primary Non-Small Cell Lung Carcinomas¹

Center for Biotechnology, Baylor College of Medicine, The Woodlands, Texas 77381 [H-J. X., S-X. H., W. F. B.]; Department of Pathology, Baylor College of Medicine, Houston, Texas 77030 [P. T. C.]; and Division of Surgical Oncology, Denver General Hospital, Denver, Colorado 80204 [G. E. M.]

Retinoblastoma (RB) protein expression was examined in paraffin and frozen tissue sections of 36 primary non-small cell lung carcinomas (NSCLC) using immunohistochemistry with confirmation by direct Western blotting. A normal RB protein staining pattern was present in 24 and absent in 10 NSCLC. Two additional RB positive primary tumors have major foci in which all tumor cells showed no RB protein staining. Significantly more high-stage (stages III and IV) NSCLC had altered RB protein expression than those with low-stage (stages I and II) tumors (P < 0.05). The results suggest that absence of the RB expression may be associated with the initiation and/or progression of many NSCLC. This is the first report of successful RB staining in paraffin sections.

¹ This study was supported by Grant EYO-2715 from the NIH and Grant 2978 from the Council for Tobacco Research, Inc.

² To whom requests for reprints should be addressed, at The Center for Biotechnology, Baylor College of Medicine, 4000 Research Forest Drive, The Woodlands, TX 77381.

Received 3/13/91. Accepted 4/ 1/91.

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