This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hermonat, P. L. Search for Related Content PubMed PubMed Citation Articles by Hermonat, P. L.

Inhibition of H-ras Expression by the Adeno-associated Virus Rep78 Transformation Suppressor Gene Product¹

Departments of Obstetrics and Gynecology, and Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205

Here the adeno-associated virus Rep78 gene product was found to inhibit the expression of the chloramphenicol acetyltransferase and the bladder cancer-derived EJ-H-ras coding sequences when they were under the control of the natural cellular H-ras regulatory sequences. However, Rep78 had little or no effect on the expression of these same coding sequences when they were under the control of the regulatory sequences of the murine osteosarcoma virus long terminal repeat. These data indicate that the inhibition of H-ras by Rep78 depends upon sequences present within the cellular H-ras upstream regulatory region. Furthermore, these and earlier data indicate that Rep78 functions as an "antion-cogene" or transformation suppressor gene, inhibiting H-ras as well as several viral oncogenes.

¹ This investigation was supported in part by American Cancer Society Institutional Grant IN-11-29 to the Johns Hopkins University.

² To whom requests for reprints should be addressed, at the Department of Obstetrics and Gynecology, Slot 518, University of Arkansas for Medical Sciences, 4301 West Markham, Little Rock, AR 72205.

Received 1/14/91. Accepted 4/12/91.

This article has been cited by other articles:

				P. G. Needham, J. M. Casper, V. Kalman-Maltese, K. Verrill, J. D. Dignam, and J. P. Trempe Adeno-associated virus rep protein-mediated inhibition of transcription of the adenovirus major late promoter in vitro. J. Virol., July 1, 2006; 80(13): 6207 - 6217. [Abstract] [Full Text] [PDF]

				J. M. Casper, J. M. Timpe, J. D. Dignam, and J. P. Trempe Identification of an Adeno-Associated Virus Rep Protein Binding Site in the Adenovirus E2a Promoter J. Virol., January 1, 2005; 79(1): 28 - 38. [Abstract] [Full Text] [PDF]

				M. Urabe, K. Kogure, A. Kume, Y. Sato, K. Tobita, and K. Ozawa Positive and negative effects of adeno-associated virus Rep on AAVS1-targeted integration J. Gen. Virol., August 1, 2003; 84(8): 2127 - 2132. [Abstract] [Full Text] [PDF]

				D. F. Lackner and N. Muzyczka Studies of the Mechanism of Transactivation of the Adeno-Associated Virus p19 Promoter by Rep Protein J. Virol., July 17, 2002; 76(16): 8225 - 8235. [Abstract] [Full Text] [PDF]

				T. Cathomen, D. Collete, and M. D. Weitzman A Chimeric Protein Containing the N Terminus of the Adeno-Associated Virus Rep Protein Recognizes Its Target Site in an In Vivo Assay J. Virol., March 1, 2000; 74(5): 2372 - 2382. [Abstract] [Full Text]

				P.-F. Su, S.-Y. Chiang, C.-W. Wu, and F. Y.-H. Wu Adeno-Associated Virus Major Rep78 Protein Disrupts Binding of TATA-Binding Protein to the p97 Promoter of Human Papillomavirus Type 16 J. Virol., March 1, 2000; 74(5): 2459 - 2465. [Abstract] [Full Text]

				D. Rinaudo, S. Lamartina, G. Roscilli, G. Ciliberto, and C. Toniatti Conditional Site-Specific Integration into Human Chromosome 19 by Using a Ligand-Dependent Chimeric Adeno-Associated Virus/Rep Protein J. Virol., January 1, 2000; 74(1): 281 - 294. [Abstract] [Full Text]

				D. Zhan, A. D. Santin, Y. Liu, G. P. Parham, C. Li, C. Meyers, and P. L. Hermonat Binding of the Human Papillomavirus Type 16 p97 Promoter by the Adeno-associated Virus Rep78 Major Regulatory Protein Correlates with Inhibition J. Biol. Chem., October 29, 1999; 274(44): 31619 - 31624. [Abstract] [Full Text] [PDF]

				J. Wu, M. D. Davis, and R. A. Owens Factors Affecting the Terminal Resolution Site Endonuclease, Helicase, and ATPase Activities of Adeno-Associated Virus Type 2 Rep Proteins J. Virol., October 1, 1999; 73(10): 8235 - 8244. [Abstract] [Full Text] [PDF]

				J. A. Chiorini, S. Afione, and R. M. Kotin Adeno-Associated Virus (AAV) Type 5 Rep Protein Cleaves a Unique Terminal Resolution Site Compared with Other AAV Serotypes J. Virol., May 1, 1999; 73(5): 4293 - 4298. [Abstract] [Full Text]

				J. A. Chiorini, F. Kim, L. Yang, and R. M. Kotin Cloning and Characterization of Adeno-Associated Virus Type 5 J. Virol., February 1, 1999; 73(2): 1309 - 1319. [Abstract] [Full Text]

				S. Weger, M. Wendland, J. A. Kleinschmidt, and R. Heilbronn The Adeno-Associated Virus Type 2 Regulatory Proteins Rep78 and Rep68 Interact with the Transcriptional Coactivator PC4 J. Virol., January 1, 1999; 73(1): 260 - 269. [Abstract] [Full Text] [PDF]

				J. A. Chiorini, B. Zimmermann, L. Yang, R. H. Smith, A. Ahearn, F. Herberg, and R. M. Kotin Inhibition of PrKX, a Novel Protein Kinase, and the Cyclic AMP-Dependent Protein Kinase PKA by the Regulatory Proteins of Adeno-Associated Virus Type 2 Mol. Cell. Biol., October 1, 1998; 18(10): 5921 - 5929. [Abstract] [Full Text]