| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Cancer Metastasis Program, Michigan Cancer Foundation, Detroit, Michigan 48201
Tumor autocrine motility factor (AMF) is a cytokine which stimulates both random and directed cell migration by self-producing cells. AMF has been detected in and purified from serum-free conditioned medium of murine B16-F1 melanoma cells. Under nonreducing conditions AMF migrates in sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band of Mr 55,000, whereas under reducing conditions it migrates as a single polypeptide of Mr 64,000. Two-dimensional polyacrylamide gel electrophoresis of the purified AMF resolved two polypeptides with isoelectric points of 6.35 (major) and 6.4 (minor). No carbohydrate side chains were detected in the B16-F1 AMF. Purified AMF stimulated B16-F1 cell migration in a dose-dependent fashion and bound directly in a protein-protein-binding assay to the AMF receptor, a cell surface glycoprotein of Mr 78,000 [glycoprotein (gp) 78]. The involvement of gp78 in AMF-stimulated function was demonstrated by motility assays. These results suggest that AMF is the natural ligand for the gp78-AMF receptor.
1 This work was supported in part by the Paul Zuckerman Support Foundation for Cancer Research to A.R.
2 Present address: Gunma University School of Medicine, Department of Orthopaedic Surgery, 3-39-22 Showa-Machi, Maebashi-Shi, Gunma-Ken, Japan.
3 Present address: Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021.
4 To whom requests for reprints should be addressed, at Meyer L. Prentis Cancer Center, 110 E. Warren Avenue, Detroit, MI 48201-1379
Received 12/19/90. Accepted 4/22/91.
This article has been cited by other articles:
|
|
 |
|
  T. Yanagawa, T. Funasaka, S. Tsutsumi, H. Hu, H. Watanabe, and A. Raz Regulation of Phosphoglucose Isomerase/Autocrine Motility Factor Activities by the Poly(ADP-Ribose) Polymerase Family-14 Cancer Res., September 15, 2007; 67(18): 8682 - 8689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. G. Jiang, A. Raz, A. Douglas-Jones, and R. E. Mansel Expression of Autocrine Motility Factor (AMF) and Its Receptor, AMFR, in Human Breast Cancer J. Histochem. Cytochem., February 1, 2006; 54(2): 231 - 241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yanagawa, T. Funasaka, S. Tsutsumi, T. Raz, N. Tanaka, and A. Raz Differential Regulation of Phosphoglucose Isomerase/Autocrine Motility Factor Activities by Protein Kinase CK2 Phosphorylation J. Biol. Chem., March 18, 2005; 280(11): 10419 - 10426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Yanagawa, T Funasaka, S Tsutsumi, H Watanabe, and A Raz Novel roles of the autocrine motility factor/phosphoglucose isomerase in tumor malignancy Endocr. Relat. Cancer, December 1, 2004; 11(4): 749 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kara, Y. Ohta, Y. Tanaka, M. Oda, and Y. Watanabe Autocrine motility factor receptor expression in patients with stage I non-small cell lung cancer Ann. Thorac. Surg., March 1, 2001; 71(3): 944 - 948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G La Riviere, J. Klein Gebbinck, M. Driessens, and E Roos Pertussis toxin inhibition of T-cell hybridoma invasion is reversed by manganese-induced activation of LFA-1 J. Cell Sci., January 3, 1994; 107(3): 551 - 559. [Abstract] [PDF]
|
|
|
|
|
|
H.-L. Cheng, M. L. Steinway, J. W. Russell, and E. L. Feldman GTPases and Phosphatidylinositol 3-Kinase Are Critical for Insulin-like Growth Factor-I-mediated Schwann Cell Motility J. Biol. Chem., August 25, 2000; 275(35): 27197 - 27204. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
