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Subtractive Cloning of a Hybrid Human Endogenous Retrovirus and Calbindin Gene in the Prostate Cell Line PC3

Cytometrics, Division of Specialty Laboratories, Inc., San Diego, California 92121

A complementary DNA clone containing human endogenous retrovirus-like sequences spliced to sequences encoding human calbindin was discovered by complementary DNA subtraction analysis between two human prostate cell lines, PC3 and DU145. This gene is presumably activated by the long terminal repeat of the retrovirus-like sequence. It belongs to a member of the retrovirus-like H (tRNA^his primer binding sequence) family. The level of this transcript is high in PC3, derived from a prostate bone metastasis, but not in DU145, derived from a prostate brain metastasis.

Received 4/30/91. Accepted 6/14/91.

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