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Departments of Thoracic Surgery [A. G. C., T. M., J. A. R.], Tumor Biology [J. A. R.], Pathology [K. R. C., J. Y. R.], and Gastrointestinal Oncology and Digestive Diseases [B. L.], The University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030
Genomic DNA was extracted from archival pathology specimens comprising 10 squamous and 14 adenocarcinomas, including 7 with Barrett's epithelium adjacent to tumor, and corresponding normal esophagus from the resection margin. The polymerase chain reaction was used to amplify selected exons of p53 which were analyzed for mutations using single-strand conformation polymorphism analysis. Mutations were localized to exon 8 for 1 adenocarcinoma and to exon 5 for 1 squamous tumor and 4 of 7 Barrett's specimens. Sequencing confirmed mutations at codons 273 (CGT
CAT; adenocarcinoma) and 176 (TGCTTC; squamous) and in Barrett's epithelium at codons 152 (CCGCTG), 155 (ACCGCC) and 175 (CGCCAC). Specimens of Barrett's epithelium from separate sites had identical p53 mutations suggesting a clonal origin. Cancers arising in mutant epithelium did not have mutations corresponding to those found in the Barrett's specimens suggesting that other events are required for tumorigenesis.
1 Supported by Grant CA45178 from the National Cancer Institute, NIH (J. A. R.), by Brown and Mathers Foundations (J. A. R.), and by a grant from the Tenneco Corp. for the Division of Surgery Core Research Facility.
2 Supported by a Fellowship from the Medical Research Council of Canada. Present address: Department of Surgery, Victoria Hospital, 375 South Street, London, Ontario, Canada N6A 4G5.
3 To whom requests for reprints should be addressed.
Received 4/29/91. Accepted 6/24/91.
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