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Antibodies to Proliferating Cell Nuclear Antigen as S-Phase Probes in Flow Cytometric Cell Cycle Analysis¹

Department of Pathology, University of Umeå, S-901 87 Umeå, Sweden

The usefulness of different anti-proliferating cell nuclear antigen monoclonal antibodies as S-phase probes in flow cytometric analysis was evaluated after various fixation procedures on human cell lines. With a newly developed detergent extraction/fixation method the monoclonal antibody PC10 acted as a selective S-phase marker. A total of 27 human hematopoietic tumors were analyzed using PC10, and all exhibited the characteristic S-phase recognition pattern. The percentage of PC10-positive cells was easily calculated and showed strong correlation to the fraction of S-phase cells determined from DNA histograms. Using alternative fixation procedures PC10, on the other hand, could recognize all actively cycling cells, a feature also observed for the monoclonal antibodies, 19A2 and TOB7.

¹ This study was supported by grants from the Swedish Cancer Society (90 1127), the Lions Cancer Research Foundation, and the Medical Faculty, University of Umeå.

² To whom requests for reprints should be addressed.

Received 3/13/91. Accepted 6/17/91.

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