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Induction by Some Protein Kinase Inhibitors of Differentiation of a Mouse Megakaryoblastic Cell Line Established by Coinfection with Abelson Murine Leukemia Virus and Recombinant SV40 Retrovirus¹

Department of Chemotherapy, Saitama Cancer Center Research Institute, Ina, Saitama 362 [Y. H., J. O-K., T. K., M. H.] and Division of Hematology, Jichi Medical School, Minamikawachi, Tochigi 329-04 [S. K., T. S., Y. M.], Japan

Mouse C1 line cells are megakaryoblastic cells established by coinfection of Abelson murine leukemia virus and recombinant simian virus 40. We examined the effects of various compounds on growth and differentiation of these cells. Megakaryocytic differentiation of C1 cells was not induced by cytokines that stimulate megakaryocytic maturation of normal progenitor cells, such as interleukin 3 and 6 and granulocyte-macrophage colony-stimulating factor. However, the cells were induced to differentiate into megakaryocytes by treatment with some protein kinase inhibitors. The inhibition of v-abl tyrosine kinase activity preceded induction of differentiation of the cells treated with tyrosine kinase inhibitors such as genistein, herbimycin A, and erbstatin. Treatment of C1 cells with a v-abl antisense oligomer inhibited their proliferation and induced acetylcholinesterase activity, a typical marker of megakaryocytic differentiation. These results suggest that inhibition of v-abl function is associated with induction of megakaryocytic differentiation of C1 cells. Among the compounds tested, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7), a potent inhibitor of cyclic nucleotide-dependent and Ca²⁺-phospholipid-dependent (protein kinase C) protein kinases, was the most potent inducer of differentiation of C1 cells. However, the differentiation-inducing effect of H-7 was unlikely to be mediated through inhibition of protein kinase C or cyclic nucleotide-dependent kinases, because other types of inhibitors of these kinases were not effective, and a protein kinase activator (phorbol ester) induced differentiation of C1 cells. Moreover, neither v-abl mRNA expression nor v-abl kinase activity in C1 cells was affected by treatment with H-7. These findings indicate that induction of megakaryocytic differentiation by H-7 is not related to inhibition of v-abl kinase, but rather to some novel function of H-7.

¹ Supported by grants from the Ministry of Education, Culture and Science and from the Ministry of Health and Welfare, Japan.

² To whom requests for reprints should be addressed, at Saitama Cancer Center Research Institute, Ina-machi, Kitaadachi-gun, Saitama-ken 362, Japan.

Received 11/ 5/90. Accepted 6/20/91.

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