This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gomm, J. J. Articles by Coombes, R. C. Search for Related Content PubMed PubMed Citation Articles by Gomm, J. J. Articles by Coombes, R. C.

Localization of Basic Fibroblast Growth Factor and Transforming Growth Factor ß₁ in the Human Mammary Gland¹

Department of Medical Oncology, Charing Cross Hospital, Fulham Palace Rd., London, W6 8RF [J. J. G., J. S., G. K. R., R. B., R. C. C.], and Department of Histopathology, The Women's Hospital, Catherine's St., Liverpool, L8 7NJ [L. T.], England

The presence and distribution of basic fibroblast growth factor (bFGF) and transforming growth factor ß₁ in benign and malignant human breast tissue were determined by immunohistochemistry and immunoblotting.

Peroxidase staining of biopsy specimens using a polyclonal antibody to amino acids 1–24 of bFGF and a monoclonal antibody to whole recombinant bFGF showed this growth factor to be localized in the myoepithelial cells of the benign breast. Epithelial cells and stroma were negative. In hyperplasia and intraductal carcinoma in situ staining was still seen around the perimeter of enlarged ducts. In malignant biopsies, however, staining was seen only when benign elements were present or residual myoepithelial cells and basement membrane remained. Antigen absorption and immunoblotting confirmed the antibody staining to be specific for bFGF.

Transforming growth factor ß₁ was shown, using the same techniques, to be located in the periductal and intraductal stroma, closely associated with epithelial or myoepithelial cells in the benign and malignant breast.

The relative localization of these two growth factors in the mammary gland may be significant in the control of breast development and/or tumor formation and progression.

¹ Supported by a Breast Cancer Research Trust Grant and a Cancer Research Campaign Grant.

² To whom requests for reprints should be addressed.

Received 7/ 2/90. Accepted 6/25/91.

This article has been cited by other articles:

				R. Korah, M. Boots, and R. Wieder Integrin {alpha}5{beta}1 Promotes Survival of Growth-Arrested Breast Cancer Cells: An in Vitro Paradigm for Breast Cancer Dormancy in Bone Marrow Cancer Res., July 1, 2004; 64(13): 4514 - 4522. [Abstract] [Full Text] [PDF]

				S L. Rosa, F Sessa, L Colombo, M G Tibiletti, D Furlan, and C Capella Expression of acidic fibroblast growth factor (aFGF) and fibroblast growth factor receptor 4 (FGFR4) in breast fibroadenomas J. Clin. Pathol., January 1, 2001; 54(1): 37 - 41. [Abstract] [Full Text] [PDF]

				R. M. Korah, V. Sysounthone, Y. Golowa, and R. Wieder Basic Fibroblast Growth Factor Confers a Less Malignant Phenotype in MDA-MB-231 Human Breast Cancer Cells Cancer Res., February 1, 2000; 60(3): 733 - 740. [Abstract] [Full Text]

				S. C. Heffelfinger, M. A. Miller, R. Yassin, and R. Gear Angiogenic Growth Factors in Preinvasive Breast Disease Clin. Cancer Res., October 1, 1999; 5(10): 2867 - 2876. [Abstract] [Full Text] [PDF]

				D. Ledoux, D. Caruelle, J.-C. Sabourin, J. Liu, M. Crepin, D. Barritault, and J. Courty Cellular Distribution of the Angiogenic Factor Heparin Affin Regulatory Peptide (HARP) mRNA and Protein in the Human Mammary Gland J. Histochem. Cytochem., September 1, 1997; 45(9): 1239 - 1246. [Abstract] [Full Text] [PDF]

				A B Glick, M M Lee, N Darwiche, A B Kulkarni, S Karlsson, and S H Yuspa Targeted deletion of the TGF-beta 1 gene causes rapid progression to squamous cell carcinoma. Genes & Dev., October 15, 1994; 8(20): 2429 - 2440. [Abstract] [PDF]

				Y Ke, D. Fernig, M. Wilkinson, J. Winstanley, J. Smith, P. Rudland, and R Barraclough The expression of basic fibroblast growth factor and its receptor in cell lines derived from normal human mammary gland and a benign mammary lesion J. Cell Sci., January 9, 1993; 106(1): 135 - 143. [Abstract] [PDF]