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Qualitative and Quantitative Changes of Human Tenascin Expression in Transformed Lung Fibroblast and Lung Tumor Tissues: Comparison with Fibronectin¹

Institute for Comprehensive Medical Science, Fujita Health University School of Medicine, Toyoake, Aichi 470-11 [F. O., K. T., K. S.], and Pathology Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo 104, [S. H., Y. S.] Japan

Qualitative and quantitative alternations of human tenascin (TN) expression in virally transformed lung fibroblasts and in lung tumor tissues were investigated using S₁ nuclease protection analysis in comparison with those of fibronectin (FN). Transformed fibroblasts and fetal lung tissues expressed more TN mRNA with an extra sequence encoding the sixth FN type III repeat than did normal cells and adult tissues. The splicing pattern of TN mRNA was also altered in many lung cancer tissues, showing increased or sometimes decreased expression of the TN mRNA with the extra sequence when compared with their surrounding normal tissues. These results provide additional evidence for the onco-developmental regulation of alternative RNA splicing in human lung tissues, first observed with FN mRNA (F. Oyama, et al., Cancer Res., 50: 1075–1078, 1990). Quantitative analysis of the levels of TN and FN mRNAs showed that the ratio of TN mRNA to FN mRNA was significantly increased in transformed fibroblasts and in some lung tumor tissues, when compared with their normal counterparts. Among different types of lung tumors, a significant increase of the TN/FN ratio was observed with most squamous cell carcinoma but with only a small fraction of adenocarcinoma. Since TN has been shown to inhibit cell adhesion to FN, the altered ratio of TN mRNA to FN mRNA may well affect the adhesive and migratory properties of tumor cells in lung cancer tissues.

¹ This work was supported by Grants-in-Aid from the Ministry of Education, Science, and Culture of Japan; a Special Coordination Fund from Science and Technology Agency of Japan; and grants from Fujita Health University and the Naito Foundation.

² Present Address: Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo, Izumi, Osaka 590-02, Japan.

³ To whom requests for reprints should be addressed.

Received 6/12/91. Accepted 7/ 8/91.

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