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Different Energy Metabolism in Two Human Small Cell Lung Cancer Subpopulations Examined by ³¹P Magnetic Resonance Spectroscopy and Biochemical Analysis in Vivo and in Vitro¹

Department of Oncology, Finsen Institute-Rigshospitalet, Blegdamsvej 9, DK-2100 Copenhagen [P. E. G. K.]; Institute of Pathological Anatomy, University of Copenhagen, Fred. d. 5. vej 11, DK-2100 Copenhagen [M. S-T.]; and Department of Biochemistry A, NMR Center, Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen [B. Q.], Denmark

Two human small cell lung cancer tumor lines, maintained as solid tumor xenografts on nude mice and as in vitro cell cultures, were studied by in vivo ³¹P magnetic resonance spectroscopy and by biochemical analysis of extracts of solid tumors and cell cultures. The tumor lines CPH SCCL 54A and CPH SCCL 54B are subpopulations from the same tumor. In solid tumors (n = 125), the ATP/P_i ratio was greater in 54A than in 54B. This was due to a higher ATP level in 54A, whereas there was no difference in P_i, ADP, and AMP. A decrease in ATP/P_i during growth was caused by a decline in ATP, whereas P_i remained unchanged. Small amounts of phosphocreatine were found in the xenografts and in tumor extracts, but not in the cell extracts; correspondingly, there was a low creatine kinase activity in solid tumors and no activity in the cell cultures. Thus, the phosphocreatine content of the solid tumors originated from the stroma. A difference in ATP content between 54A and 54B was also found in cell cultures; hence, the metabolic difference is an intrinsic quality of the malignant cells and is not caused by the host system.

¹ This study was supported by the Danish Medical Research Council, Grants 12-8817 and 12-9867; the Danish Cancer Society, Grant 90-039; and the Danish Hospital Foundation for Medical Research, Grant 40/89.

² To whom requests for reprints should be addressed.

Received 3/19/91. Accepted 7/17/91.

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