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Frequent Allelic Losses and Mutations of the p53 Gene in Human Ovarian Cancer¹

National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104 [A. O., Y. S., T. S., M. T., J. Y.]; Department of Obstetrics and Gynecology, the Jikei University School of Medicine, 25-8, Nishi-Shinbashi 3-chome, Minato-ku, Tokyo 105 [S. Y., Y. T.], Japan

The p53 gene on chromosome 17p is considered to be a tumor suppressor gene, and frequent mutations of the p53 gene have been found in a wide variety of human cancers. We examined 31 ovarian cancers for allelic losses and mutations of the p53 gene by polymerase chain reaction-single strand conformation polymorphism analysis as well as restriction fragment length polymorphism analysis. Allelic loss of the p53 gene was detected in 16 of 20 cases (80%). Mutations were detected in 9 of 31 cases (29%): 2 cases in exon 4; 5 cases in exons 5–6; and 2 cases in exons 7–8. In 8 of 9 cases, p53 mutations were accompanied by losses of the normal allele. These alterations of the p53 gene were commonly detected from stage I to stage IV. These results suggest that alterations of the p53 gene play an important role in the development of human ovarian cancers.

¹ This work was supported in part by a Grant-in-Aid for a Comprehensive 10-Year Strategy for Cancer Control from the Ministry of Health and Welfare of Japan and by Grants-in-Aid from the Ministry of Health and Welfare and the Ministry of Education, Science, and Culture of Japan. Y. S. is an awardee of a Research Resident Fellowship from the Foundation for Promotion of Cancer Research.

² To whom requests for reprints should be addressed, at Section of Studies on Metastasis, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104, Japan.

Received 4/16/91. Accepted 7/19/91.

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